粒用型和菜用型大豆鲜籽粒的比较转录组学分析OA

In this study,we analyzed the gene expression differences between fresh seeds of grain and vegeta-ble soybean to providing theoretical basis and technical support for the mechanism of soybean fresh seed quality for-mation by transcriptome sequencing.High throughput sequencing technology(IlluminaHiSeq 4000)was used to per-form transcriptome sequencing on R6 stage fresh seeds of the grain soybean variety Fudou 234 and the vegetable soybean variety Mindou 7.A total of 39.92 Gb sequencing data were obtained,representing 56,956 annotated genes.Among them,932 new genes and 2088 differentially expressed genes(DEGs)were annotated.Taking Fudou 234 as a reference,918 DEGs were upregulated in Mindou 7.Among 918 upregulated genes,GO analysis revealed that genes related to biological processes such as glutamine metabolism,(1-＞3)-β-D-glucan biosynthesis,and carbohy-drate metabolism were significantly enriched;Genes related to galactose metabolism,lignin,lignin and wax biosyn-thesis,starch and sucrose metabolism,and other pathways were significantly enriched in the KEGG pathway.Simi-larly,1170 genes were downregulated in Mindou 7,and GO analysis showed significant enrichment of genes related to biological processes such as endoplasmic reticulum unfolded protein response,xyloglucan metabolism,and cell wall biogenesis in biological processes;Genes related to pathways such as ABC transporters,GPI anchored protein biosynthesis,and protein processing in the endoplasmic reticulum were significantly enriched in the KEGG path-way.Among the 48 DEGs related to starch and sucrose metabolism,25 were upregulated in the fresh seeds of grain soybean Fudou 234 and 23 were upregulated in the fresh seeds of fresh soybean Min Dou 7.At the same time,qRT-PCR technology was used to verify the reliability of transcriptome sequencing.This study will provide a theoretical basis for improving the quality of soybean fresh seeds.