甘蓝型油菜GSK基因家族的全基因组鉴定及核盘菌诱导应答分析OA

To elucidate the functional mechanisms of the GSK(glycogen synthase kinase)gene family in dis-ease resistance in rapeseed(Brassica napus L.),this study aims to elucidate the response dynamics of this gene fami-ly to Sclerotinia sclerotiorum infection through genome-wide identification of its members combined with bioinfor-matic analyses and molecular experiments.Members of the GSK family were identified from the rapeseed genome(cv.ZS11)using BlastP and HMMER methods.A phylogenetic tree was constructed using MEGA7.Expression pat-terns were analyzed based on transcriptome sequencing(at 48 hpi with S.sclerotiorum)and qRT-PCR validation.The results showed that a total of 35 GSK gene family members were identified in B.napus genome,designated as BnaSKs.All members contained the conserved STKc_GSK3 kinase domain.Phylogenetic analysis classified them into four subfamilies(Group Ⅰ-Ⅳ).Collinearity analysis revealed the evolutionary dynamics of the B.napus genome and its phylogenetic relationships with related species.Promoter regions are enriched with hormone-responsive(ABA,JA)and stress-responsive cis-elements,suggesting they are involved in rapeseed resistance regulation.Tis-sue-specific expression analysis indicated functional divergence among BnaSK subfamily members across different developmental stages and organs:Group Ⅰ members(e.g.,BnaSK1.5,BnaSK1.6)are highly expressed in leaves;Group Ⅱ showed preferential expression in leaves and stems;Group Ⅲ are specifically expressed in pollen;and Group Ⅳ are widely expressed across various tissues.Following S.sclerotiorum infection(48 hpi),Group Ⅰ members(BnaSK1.2,BnaSK1.4,BnaSK1.5 and BnaSK1.6)are significantly upregulated(P＜0.01),with BnaSK1.2 and BnaSK1.4 expression reaching four-fold relative to controls,only a few genes in Group Ⅱ are slightly up-regulated,while Group Ⅳ exhibited no significant changes.These results confirmed that Group Ⅰ members,particularly BnaSK1.2 and BnaSK1.4,were likely involved in the early defense response against S.sclerotiorum infection.This study systematically revealed the evolutionary characteristics of the GSK gene family in B.napus and its expression dynamics under S.sclerotiorum induction,providing key candidate genes for rapeseed disease resistance breeding.