590nm LED光照射对玫瑰痤疮样小鼠皮损血管内皮细胞CD31表达的影响OA
Effect of 590 nm LED light irradiation on the expression of CD31 in vascular endothelial cells of rosacea-like skin lesions in mice
目的 探讨590 nm LED光照射对玫瑰痤疮样皮炎小鼠皮损血管内皮细胞CD31表达的影响.方法 将雌性BALB/c小鼠随机分为对照组(PBS)、模型组(LL-37)和治疗组(LL-37+LED 590 nm光),每组5只.PBS组背部皮内注射40 μl PBS溶液,其余各组小鼠予背部注射等量抗菌肽LL-37构建玫瑰痤疮小鼠模型(每12 h注射1次,共4次).造模成功后治疗组小鼠给予590 nm LED光治疗,观察皮肤变化,治疗结束后取背部皮肤组织通过免疫组化法检测CD31蛋白表达.结果 与PBS组相比,LL-37组中CD31蛋白表达量显著升高,差异有统计学意义(P<0.05),与LL-37组相比,590 nm LED组中表达量显著降低,差异有统计学意义(P<0.05),CD31阳性毛细血管数量在模型组中显著增加、治疗组中减少.结论 590 nm LED光可能通过抑制玫瑰痤疮皮损血管内皮细胞CD31的表达从而达到改善血管扩张、抑制血管增生的作用.
Objective To investigate the effect of 590nm LED light irradiation on the expression of CD31 in vascular endothelial cells of rosacea-like skin lesions in mice.Methods Female BALB/c mice were randomly assigned to either a control group(PBS)or an experimental group(LL-37)or treatment group(LL-37+LED 590 nm light),with 5 mice in each group.The PBS group received an intradermal injection of 40 μl PBS solution on the back,while mice in the other groups were injected with an equal volume of the antimicrobial peptide LL-37 to establish a rosacea mouse model(injections were administered every 12 hours for a total of 4 times).After successful model establishment,mice in the treatment group were treated with 590nm LED light.Skin changes were observed,and after treatment completion,dorsal skin tissue was collected.By immunohistochemistry method,we assessed The expression of CD31 protein.Results Compared with the PBS group,the expression level of CD31 protein was significantly increased in the LL-37 group(P<0.05).Compared with the LL-37 group,the expression level was significantly decreased in the 590 nm LED group(P<0.05).Conclusion 590 nm LED light may improve telangiectasia and inhibit angiogenesis in rosacea lesions by suppressing the expression of CD31 in vascular endothelial cells.
热伊莱·阿皮孜;周丹;刘淑珍;贾琳
新疆医科大学第一附属医院,新疆 乌鲁木齐,830013新疆医科大学第一附属医院,新疆 乌鲁木齐,830013新疆医科大学第一附属医院,新疆 乌鲁木齐,830013新疆医科大学第一附属医院,新疆 乌鲁木齐,830013
玫瑰痤疮590 nm LED光CD31
rosacea590 nm LED lightCD31
《中国医疗美容》 2026 (4)
29-33,5
新疆医科大学第一附属医院青年科研启航基金(2023YFY-QKQN-71)
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