西瓜雄花两性花同株性状的遗传分析及基因定位OA

Cucurbitaceae crops,which exhibit all seven sexual types found in angiosperms,have become ideal model or-ganism for investigating the mechanisms of plant sex determination.The primary sexual type in watermelon is monoe-ciousr,while certain germplasm lines exhibit the andromonoecious sexual type,wherein both male and hermaphroditic flowers are produced simultaneously on the same watermelon plant.To investigate the sex determination mechanism in watermelon,the andromonoecious germplasm WM254 and normal monoecious germplasm WT2 were used as experimen-tal materials.By constructing an F2 segregating population,it was discovered that the andromonoecious trait in watermel-on is controlled by a recessive single gene.Through a combination of BSA-seq sequencing and linkage analysis within the F2 population,the gene responsible for the andromonoecious trait in watermelon was preliminarily localized to a re-gion between 29.45 Mb and 29.58 Mb on chromosome 3.This interval contains 14 candidate genes,several of which are potentially related to sexual differentiation.Based on this candidate region,a molecular marker tightly linked to the andro-monoecious trait in watermelon was developed.The findings of this study lay a foundation for further fine mapping of the gene regulating andromonoecy in watermelon and subsequent functional validation,while also providing a reference for research on sex type differentiation in Cucurbitaceae species.