基于GWAS分析挖掘甜瓜熟性相关候选基因OA

To identify the genetic loci and candidate genes associated with melon maturity traits and thereby accelerate the breeding of novel early-maturing melon cultivar,in this study,151 muskmelon accessions displaying diverse maturity characteristics were analyzed using a whole-genome re-sequencing approach to perform a genome-wide association stud-ies(GWAS)on maturity-related traits.The primary objective was to detect significantly associated loci,thereby establish-ing a theoretical basis for the development of early-maturing melon cultivar.The results indicate that among the 151 ana-lyzed accessions,36 exhibited early maturity characteristics.A total of 2 165 342 high-quality SNPs loci were identified,and the MLM(QK)model identified 55 SNP loci significantly associated with melon ripening traits.Within the 100 kb flanking regions surrounding these loci,a total of 240 alleles were identified.Integrated functional annotation and enrich-ment analyses revealed that the candidate genes are predominantly involved in phenylpropanoid biosynthesis,secondary metabolite biosynthesis,fatty acid metabolism,amino acid biosynthesis,and plant hormone signal transduction pathways.The two most significant SNP loci are located on chromosomes 4 and 12,respectively.The genes closely associated with the SNP locus on chromosome 4 are MELO3C009181 and MELO3C009179,whereas the gene linked to the SNP locus on chromosome 12 is MELO3C002391.Notably,MELO3C009181(LAX2)encodes an auxin transporter-like protein,and MELO3C002391(PER7)encodes a peroxidase,both of which represent potential candidate genes involved in the regula-tion of melon ripening.This study establishes a molecular foundation for ripening-related breeding and the development of early-maturing melon cultivar.