盐酸氯苯胍遗传毒性评价研究OA

Robenidine hydrochloride(ROBH)is a chemically synthesized anticoccidial drug widely used in animal husbandry because of its good efficacy and relatively low production cost.At present,information on the potential genotoxicity of ROBH is limited and remains highly controversial.To clarify the genotoxicity of ROBH,the Ames test,mouse bone marrow cell micronucleus test,and single-cell gel electrophoresis assay were employed to investigate its genotoxic effects at the gene,chromosome,and DNA levels,respectively.The results showed that,compared with the sterile distilled water group,the average numbers of mutant colonies in the low-dose ROBH groups(200,40,and 8 μg/plate)of the four test strains were not significantly different(P>0.05),whereas the average numbers of mutant colonies in the high-dose ROBH groups(5 000 and 1 000 μg/plate)were significantly decreased(P<0.05).Compared with the Negative control group,the micronucleus rates of polychromatic erythrocytes(PCEs)in the 195.00 and 97.50 mg/(kg·bw)ROBH dose groups were significantly increased(P<0.01).Obvious comet tailing was observed in hepatocellular carcinoma cell line 2(HepG2)cells treated with 20 and 25 μmol/L ROBH.Compared with the Control group,the percentages of tail DNA in HepG2 cells in the 20 and 25 μmol/L ROBH groups were significantly increased(P<0.01).These results indicate that low-dose ROBH is non-mutagenic to the test strains,whereas high-dose ROBH shows bacterial toxicity to the test strains.ROBH can induce chromosomal damage and DNA strand breaks in mammalian cells,demonstrating clear genotoxicity.The findings of this study provide important evidence for the re-evaluation of the safety of ROBH.