鸽源沙门菌的分离鉴定及其耐药性和毒力基因分析OA

A suspected Salmonella infection occurred in a large-scale pigeon farm in Hebei Province.To identify the pathogen and characterize its drug resistance and virulence profiles,liver tissues from dead pigeons were subjected to bacterial isolation and culture.The isolates were identified through morphological identification,biochemical tests,serotyping,and 16S rRNA gene sequencing.Drug susceptibility testing and whole-genome sequencing were further performed to analyze the resistance phenotype,drug resistance genes,and virulence genes of the isolate.The results showed that one strain of Salmonella typhimurium was isolated.Drug susceptibility testing indicated that the isolate was resistant to neomycin and streptomycin.Whole-genome sequencing analysis revealed that the isolate carried 22 drug resistance genes and 137 virulence genes.These results indicate that a pigeon-derived Salmonella typhimurium strain carrying multiple drug resistance and virulence genes was successfully isolated in this study,providing a reference for the prevention,control,medication,and further research on pigeon-derived Salmonella typhimurium.