茅苍术地上部分总黄酮纯化工艺及抑菌活性研究OA

This experiment used the aerial part of Atractylodes lancea as the material,total flavonoids were extracted by ultrasonic method.Then the optimal resin model was selected based on the results of static adsorption and static desorption experiments;Using adsorption rate and desorption rate as indicators,the effects of sample concentration,sample loading speed,sample loading volume,and eluent concentration,elution speed,and eluent volume on the purification process of total flavonoids were investigated,and the total flavonoid purification process was optimized through orthogonal design.In addition,the antibacterial activities of total flavonoids against Staphylococcus aureus and Escherichia coli were studied using the agar plate method.The results showed that D101 macro-porous resin was the most suitable one.The purification process involved a sample concentration of 1.20 mg/mL,a sample loading rate of 1 BV/h,a sample volume of 30 mL,and 60 mL of 80%ethanol as the eluent.The elution rate was controlled at 1 BV/h.Using this purification condition,the total flavonoid purity could reach 48.2%.The total flavonoids obtained had a significant antibacterial effect on Staphylococcus aureus,with a minimum inhibitory concentration of 2.26 mg/mL.