总溶解气体过饱和对似鲇高原鳅生理功能的影响OA

During flood discharge,total dissolved gas(TDG)supersaturation downstream of dams can cause gas-bubble disease in fish and has become a globally ecological concern.However,the impact of this condition on fish physiology remains unclear.In this study,we systematically examined the histopathology,serum biochemistry,transcriptomes and metabolites of Triplophysa siluroides exposed to 116%TDG.T.siluroides exhibited severe gill pathology,elevated catalase(CAT)and aspartate aminotransferase(AST)activities,up-regulation of cytochrome,immune(cd48 and cd151)and glycolytic(phosphoglycerate mutase2)genes,and global depletion of glycerophospholipids.Integrated transcriptomic and metabolomic analyses revealed that differentially expressed genes and metabolites were co-enriched in three biological pathways:pyrimidine metabolism,amino acid biosynthesis,and steroid hormone biosynthesis.The results of this study demonstrate that TDG supersaturation induces physiological damage in T.siluroides,triggers an oxidative stress response,and alters the expression of downstream regulatory genes.These findings contribute to a deeper understanding of the molecular mechanisms underlying fish responses to TDG supersaturation stress and provide essential data for developing effective fish conservation strategies and ecological flow management practices.