牛支原体检测技术的研究进展OA

Bovine mastitis is a major disease constraining the healthy development of the dairy industry.Mycoplasma bovis,as one of its key pathogens,is characterized by high contagiosity,strong pathogenicity,and a lack of effective treatments,making its rapid and accurate detection crucial for prevention and control.This paper systematically reviewed the research progress in detection techniques for Mycoplasma bovis,primarily including traditional microbial isolation and culture,serological testing,and DNA-based molecular detection technologies.The advantages and limitations of various methods were analyzed.It is highlighted that molecular detection technologies—especially qPCR—demonstrate significant superiority in sensitivity and specificity,while emerging isothermal amplification technologies such as RPA and LAMP show great potential for field application due to their simplicity,rapidity,and low equipment dependency.This review aimed to provide theoretical support and technical references for the early diagnosis and integrated prevention and control of bovine mastitis.