2021-2024年北京市朝阳区临床分离肺炎克雷伯菌耐药表型及全基因组序列分析OA

This study was aimed at understanding the drug sensitivity of Klebsiella pneumoniae clinical isolates from hospitalized patients,and analyzing their drug resistance phenotypes,drug resistance genes,and high virulence genes.A total of 115 K.pneu-moniae strains clinically isolated from Chaoyang District,Beijing,from 2021 to 2024 were collected for drug sensitivity testing and whole genome sequencing.The original data were analyzed in the Microobench pathogen analysis workstation(without reference splic-ing).Statistical analysis was conducted in SPSS 27.0 software.The resistance rate of K.pneumoniae increased each year,and the resis-tance rates to 19 types of antibiotics exceeded 50%.The detection rate of carbapenem-resistant Klebsiella pneumoniae(CRKP)was 58.26%.Molecular typing revealed that the CC258 clone complex was the most prevalent sequence type in this region(45.22%),and all were ST11-type strains.A total of 14 strains of highly virulent K.pneumoniae(hvKP)were found,three of which were hyperviru-lent carbapenem-resistant K.pneumoniae(HCKP).The drug resistance of K.pneumoniae showed a continually increasing trend,and the emergence of HCKP suggested a need for vigilance regarding the evolutionary risks of"superbugs."Hospitals should strengthen clinical rational drug use,and integrate molecular monitoring and precise intervention to curb the spread of drug-resistant clones.