口腔样本及其分离的念珠菌中幽门螺杆菌特异性基因的检测OA

This study analyzed oral detection of Helicobacter pylori(Hp)through Hp-specific nucleic acid testing on oral samples from patients at the Department of Gastroenterology at our institution.The presence of Hp-specific genes in Candida isolated from oral samples was investigated.Oral samples of 200 gastroenterology patients who underwent 13C-urea breath testing(13C-UBT)for Hp were collected and cultured for Candida isolation.Hp-specific nucleic acids in the oral samples and Candida samples were de-tected.Of the 200 included patients,93 and 107 had positive and negative 13C urea breath test results,respectively.The positivity rates were 22.83 for the Hp-specific 16S rDNA gene in oral samples and 20.33%for the ureA gene.The rate of 16S rDNA and ureA double positivity was 9.33%.The detection rates of Hp in different parts of the oral cavity showed no statistically significant differences(χ2=1.930,P>0.05).The detection rate of oral Hp was significantly higher in the gastric Hp-positive group than the gastric Hp-negative group(χ2=20.480,P<0.05).The detection rate of Candida in oral samples was 10.5%,and Candida albicans showed the high-est rate among species.The positivity rate of Hp-specific 16S rDNA in samples isolated from Candida in oral samples cultured by pas-saging to the 5th generation was 39.68%;that of ureA was 52.38%;and that of both Hp 16S rDNA and ureA was 14.29%.Therefore,Hp-specific genes were detected in both oral samples and oral Candida,and gastric H.pylori infection increased the detection rate of oral H.pylori-specific genes.