2种卡介苗冻干疫苗的制备及其免疫原性的比较OA

Two types of BCG freeze-dried vaccines were prepared using different cultivation methods,and the immunogenicity of the two vaccines was compared.BCG strain was inoculated into Sutton medium and 7H9 complete medium respectively.BCG bacterial film vaccine(BCG-BV)and BCG bacterial liquid vaccine(BCG-BLV)were prepared by freeze-drying.BALB/c mice were immu-nized subcutaneously with the two vaccines,and the sera antibody levels were detected by ELISA.The lung T cell subsets of mice were detected by flow cytometry.The cytokines and inflammatory factors secreted by splenocytes stimulated in vitro were detected by ELISA.BCG-BLV contained a higher number of viable bacteria than BCG-BV.BCG-BLV induced an increase in the antibody level against Ag85B,and the subclass levels also showed a significant increase.BCG-BV induced a significant increase in the percentage of CD8+T cells in lung,whereas BCG-BLV decreased it.Both BCG-BV and BCG-BLV groups could induce the secretion of Th1(IFN-γ,IL-2)and Th2(IL-10)cytokines,and the levels of BCG-BLV immunized group were higher.Both BCG-BV and BCG-BLV could in-duce IL-1β,TNF-α and IL-6,which were characteristic cytokines of training immunity,and the BCG-BLV group induced a signifi-cantly higher level of IL-1β release.Different cultivation methods significantly influenced the immunogenicity of BCG.The freeze-dried BCG-BLV vaccine prepared in the enriched medium exhibited stronger immunogenicity.