坦布苏病毒的基因型特征分析OA

This study was aimed at isolating and identifying Tembusu virus(TMUV)from mosquito samples collected a cattle farm in the Shanghai region on August 7 and 8,2024,and systematically analyzing their genotypic characteristics,genetic diversity,host adaptability,and molecular epidemiological features,to provide a scientific basis for viral surveillance and early warning.Culex tritaeniorhynchus mosquito samples were collected and subjected to RT-qPCR detection,cell culture,PCR amplification,Sanger se-quencing,and metagenomic sequencing to identify TMUV strains and obtain full genome sequences.A total of 177 reference se-quences from the NCBI database were included for comparative analysis.Open reading frame(ORF)regions were extracted.Phyloge-netic analysis,nucleotide and amino acid similarity analysis,sliding window analysis,and amino acid mutation profiling were per-formed to evaluate genetic divergence and biological characteristics among genotypes.The isolated TMUV strain from C.tritaeniorhyn-chus mosquitoes belonged to the cluster 3.2 genotype.Phylogenetic analysis revealed that TMUV could be divided into six genotypes:old lineage,cluster 1,cluster 2.1,cluster 2.2,cluster 3.1,and cluster 3.2.Significant differences in host adaptability,geographic distribution,and molecular characteristics were observed among genotypes.Cluster 3.2 exhibited the highest host diversity.Numerous genotype-specific amino acid mutations were identified,particularly in the E(envelope)protein,in which potential key sites such as E-150N,E-365P,and E-369G may be associated with changes in viral biological properties.TMUV was found to exhibit genotypic differentiation,host expansion,and regional specificity.Specific amino acid mutations might drive phenotypic diversity and interspe-cies transmission potential.This study provides critical insights into the genetic evolution of TMUV,and offers valuable guidance for surveillance and control strategies.