1株麦氏弧菌噬菌体vB_VmeS_MHP的分离鉴定及其基因组特征分析OA

This study developed novel strategies for controlling Vibrio metschnikovii infections and phage-based formulations.Mi-gratory bird-derived Vibrio metschnikovii VM4F served as the host strain.Lytic phages were isolated and purified from lake water samples collected from the host habitat via the double-layer plate method.Subsequently,biological characterization and whole-genome bioinformatics analysis were conducted on the isolated phages.A Vibrio metschnikovii-specific lytic phage was successfully ob-tained and designated as vB_VmeS_MHP.The optimal multiplicity of infection(MOI)of this phage was determined to be 0.001,its lytic cycle was measured as 110 minutes,and the burst yield was calculated to be 404 PFU/cell.This phage demonstrated strong stress tolerance:it withstood heat at 60 ℃,acidic/alkaline conditions ranging from pH 5 to 12,and 235.7 nm UV irradiation for 90 minutes.In vitro bacteriostatic activity persisted for 6 hours,and it exhibited significant biofilm-disrupting effects.Morphological observations revealed that the phage had a head diameter of(59.3±1.8)nm and a tail length of(207.5±0.5)nm.The phage was classified within the family Siphoviridae of the order Caudovirales.Whole-genome analysis indicated that the phage possessed a linear double-stranded DNA genome with a length of 41 255 bp and a GC content of 43.48％.No virulence or antibiotic resistance genes were detected in its genome.Phylogenetic analysis suggested shared evolutionary relationships between this phage and phages from multiple regions and hosts,thus implying that cross-host jumping and recombination-driven evolution might have occurred.This study confirmed that vB_VmeS_MHP possesses excellent antibacterial efficacy and environmental adaptability,and it provides crucial theoretical support for the development of Vibrio metschnikovii phage-based control products.