基于D-loop和Cyt-b基因的黑线姬鼠种群遗传结构与汉坦病毒感染关联性分析OA
Association between Apodemus agrarius population genetic structure,according to the D-loop and Cyt-b genes,and hantavirus infection
目的 探究湖南地区黑线姬鼠种群的遗传结构及汉坦病毒感染与宿主遗传变异的关联性.方法 以黑线姬鼠肺组织总DNA为模板,基于其线粒体D-loop区及Cyt-b基因的保守区设计引物进行PCR扩增,并对扩增产物进行测序.利用MEGA 6.0和DnaSP 5.0软件分析序列特征及遗传多样性,并采用邻接法(NJ)和最大似然法(ML)构建系统发育树,以揭示种群遗传分化及其与汉坦病毒感染关联性.结果 D-loop区(658 bp)和Cyt-b基因(871 bp)的A+T含量分别为60.9％和60.7％,均高于C+G含量;检测到变异位点分别为38个和40个,对应11种和9种单倍型,单倍型多样性(H)分别为0.956和0.912,核苷酸多样性(π)为0.012 78和0.009 51,Tajima's D值为-1.446 26和-1.555 71.系统发育分析显示,浏阳种群(除LY-029-HTAA-7、LY-031-HTAA-9外)形成单系分支,双峰种群SF-014-HTAA-7独立分布;未感染样本LY-03-UAA-6与感染样本LY-016-HTAA-6在Cyt-b树中紧密聚类.结论 湖南黑线姬鼠种群具有一定程度的遗传多样性,但汉坦病毒感染与线粒体基因组变异无直接关联,提示后续研究需整合宿主生态行为与环境因素,从而解析病毒传播机制.
This study was aimed at elucidating the genetic structure of Apodemus agrarius populations in Hunan,China,and its association with hantavirus infection.Total DNA extracted from rodent lung tissues served as the template for PCR amplification of con-served mitochondrial D-loop and Cyt-b gene regions,and was followed by sequencing.Sequence characteristics and genetic diversity were analyzed in MEGA 6 and DnaSP 5 software,and phylogenetic trees were constructed with the neighbor-joining(NJ)and maxi-mum likelihood(ML)methods to assess population differentiation and virus-host linkages.Both genes showed AT-rich composi-tions[60.9％for D-loop(658 bp);60.7％for Cyt-b(871 bp)].A total of 38 and 40 variable sites were detected,corresponding to 11 and 9 haplotypes.The haplotype diversity(H)values were 0.956 and 0.912,and the nucleotide diversity(π)was 0.012 78 and 0.009 51.Tajima's D values were-1.446 26 and-1.555 71.Phylogenetic analysis indicated that most Liuyang populations(except LY-029-HTAA-7 and LY-031-HTAA-9)formed a monophyletic clade,whereas the Shuangfeng population(SF-014-HTAA-7)clustered in-dependently.Notably,an uninfected sample(LY-03-UAA-6)and an infected sample(LY-016-HTAA-6)were closely grouped in the Cyt-b tree.This study revealed that A.agrarius populations in Hunan exhibited notable genetic diversity,and no direct correlation was observed between hantavirus infection and mitochondrial genome variation.Our findings highlight the need to integrate host eco-logical behavior and environmental factors in future studies to elucidate virus transmission mechanisms.
熊亚茹;蔡亮;何方玲;刘荣娇;王娟;贾华云;湛志飞;戴志辉;杨子琪;陈生宝
湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153湖南省疾病预防控制中心(湖南省预防医学科学院),微生物分子生物学湖南省重点实验室,长沙 410153
医药卫生
汉坦病毒遗传多样性共进化D-loop区Cyt-b基因
hantavirusgenetic diversitycoevolutionD-loop regionCyt-b gene
《中国人兽共患病学报》 2026 (3)
276-284,9
国家科技重大专项(No.2018ZX10713002)湖南省自然科学基金(No.2024JJ9472,No.2021JJ70010)湖南省卫健委高层次人才支持项目(No.20240304043) National Major Project of Science and Technology(No.2018ZX10713002)Natural Science Foundation of Hunan Prov-ince(No.2024JJ9472,No.2021JJ70010)High-level Talent Support Project of Hunan Provincial Health Commission(No.20240304043)
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