2023年山东省蚊虫中部分蚊媒病毒携带情况分析OA

To understand mosquito-borne arbovirus infections and distributions in mosquitoes,we conducted mosquito surveil-lance at 6 sentinel areas in Shandong Province in August 2023.Mosquitoes were collected with handheld aspirators or ultraviolet light traps,morphologically identified,and counted.Approximately 25-50 mosquitoes were pooled per sample,homogenized,and sub-jected to total RNA extraction.RNA of the following eight mosquito-borne viruses was detected with real-time PCR:Culex flavivirus(CxFV),Kadipiro virus(KDV),Bannavirus(BAV),Getah virus(GETV),Tahyna virus(TAHV),Liao ning virus(LNV),West Nile virus(WNV),and Dengue virus(DENV).A total of 14 813 mosquitoes were collected and were identified as 6 species,among which Armigeres obturbans(6 721,45.37％),Culex tritaeniorhynchus(5 966,40.28％),and Culex pipiens pallens(1 851,12.50％)were the most common.Of the 360 pools of mosquitoes,40 pools were positive for KDV RNA:16 pools of Culex tritaeniorhynchus,18 pools of Armigeres obturbans,3 pools of Culex pipiens pallens,2 pools of Anopheles sinensis,and 1 pool of Culex quinquefasciatus.Eight pools were positive for CxFV RNA:3 pools of Culex tritaeniorhynchus,3 pools of Culex pipiens pallens,and 2 pools of Armigeres obturbans.The overall prevalence of KDV infection was 2.70 per 1 000 mosquitoes(Ct:24.57-37.03),and the CxFV infection rate was 0.54 per 1 000 mosquitoes(Ct:16.90-35.00).Mosquitoes in Shandong Province carried KDV and CxFV.Enhanced surveillance of KDV and CxFV will be essential to support the prevention and control of mosquito-borne viruses.