白蜡树ARF基因家族的鉴定与分析OA

To study ARF genes in Fraxinus chinensis,the full-length transcriptome of leaf and bark of Fraxinus chinensis was as material,and ARF genes were identificated and the systematic bioinformatics analysis and codon bias analysis were performed.The results showed that 28 ARF genes were identified,with amino acid sequence varying from 384(FcARF22)to 1 107 aa(FcARF4),isoelectric point of 5.19～8.61,aliphatic index of 63.94～79.41,all were unstable hydrophilic proteins,and located in nucleus.The prediction of protein secondary structure showed that the proportion of irregular curl was the highest,followed by α-helix and extended chain structure,and the proportion of β-corner was the lowest.The phylogenetic analysis divided 28 FcARF genes into 4 classes,including 10,9,6 and 3 members,respectively.FcARF2 and FcARF4 had significantly higher expression levels in leaves than those in barks,FcARF6,FcARF8,FcARF9,FcARF10,FcARF11,FcARF13,FcARF16,FcARF22,FcARF25,FcARF26 and FcARF27 had significantly higher expression levels in barks than those in leaves.The GC content of FcARF codons was 43.10％～49.30％,and the third base of the codon showed T3(43.45％)>A3(33.89％)>G3(26.11％)>C3(21.15％),average effective number of codon value was 53.61.The optimal codons were screened including UUC、UCC、UAC、AAA、GAG、CGG、AGA and GGA.The heterologous receptors suitable for FcARF expression were Arabidopsis thaliana,Nicotiana tabacum and Saccharomyces cerevisiae.The main factor affecting codon bias was natural selection,and mutation pressure had a certain effect.Above results provided scientific basis for further research on the heterologous transformation and gene function of FcARF genes in Fraxinus chinensis.