基于微生物-活性物质关联性的生乳保鲜期研究OA
Research on Predicting Shelf Life of Raw Milk Based on the Association between Microorganisms and Active Substances
为探究生乳储存过程中微生物增殖与活性物质降解的关联性,本研究选取20头健康奶牛的乳样,采用原奶细菌体细胞快速检测一体机监测72h内微生物动态变化,并通过ELISA试剂盒定量分析乳铁蛋白(LF)和乳过氧化物酶(LPO)的活性.结果表明,在2～8℃条件下随储存时间延长,微生物繁殖导致牛奶新鲜度显著下降(P<0.05).乳铁蛋白活性在32h后开始降解,48h平均活性降至初始值的86%,72h后进一步降至61%;乳过氧化物酶活性呈现先升高后降低的趋势.相关性分析证实,微生物数量与两种活性物质的降解率呈显著正相关(r>0.8).本研究揭示了生乳储存中活性物质的时效性衰减规律,为优化乳品保鲜工艺及质量控制提供了新的理论依据.
To investigate the correlation between microbial proliferation and degradation of active substances in raw milk during storage,milk samples from 20 healthy cows were analyzed using an integrated rapid detection system for bacterial somatic cells to monitor microbial dynamics over 72 hours,while ELISA kits quantified the activities of lactoferrin(LF)and lactoperoxidase(LPO).Results showed that microbial growth significantly reduced milk freshness(P<0.05).LF activity began to decline after 32 hours,with average activity decreasing to 86%at 48 hours and 61%at 72 hours.LPO activity exhibited an initial increase followed by a decrease.Correlation analysis confirmed a significant positive relationship between microbial count and degradation rates of both active substances(r>0.8).This study reveals the time-dependent degradation patterns of bioactive components in raw milk,providing a theoretical basis for optimizing dairy preservation strategies and quality control.
李照;陈云;刘宇;刘云鹏;董雪娜;王俊燕;王彩霞;母智深
内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特 011517内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特 011517内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特 011517内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特 011517内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特 011517内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特 011517内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特 011517内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特 011517
轻工纺织
储存时间新鲜度菌落总数乳铁蛋白乳过氧化物酶
Storage timeFreshnessTotal number of coloniesLactoferrinLactoperoxidase
《中国奶牛》 2026 (4)
42-46,5
2024年内蒙古自治区乳品营养健康与安全企业重点实验室项目(2025KYPT0065)牛乳和乳铁蛋白分离纯化关键技术研究及产业化制备(2025TEL29).
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