宁夏某规模化奶牛场牛舍环境中常见病毒的检测与分析OA
Detection and Risk Analysis of Four Viruses in the Environment of a Large-Scale Dairy Farm in Ningxia
牛奶产业是宁夏"六特"重点产业之一,随着养殖规模的不断扩大,奶牛疫病阳性率居高不下,有必要打造规模化奶牛场生物安全防控体系.本研究采用ELISA方法对宁夏某规模化奶牛场随机采集的100份奶牛血液样本进行几种奶牛常见病毒的检测,同时采集不同牛舍内的粪污、环境拭子样品进行PCR检测.结果显示,该场奶牛存在牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)、牛冠状病毒(Bovine coronavirus,BCoV)、牛轮状病毒(Bovine rotavirus,RV)和牛传染性鼻气管炎病毒(Bovine infectious rhinotracheitis virus,IBRV)感染,且这四种病毒在不同牛群圈舍环境中长期存在.本研究结果可为规模化奶牛养殖场重要疫病风险监测体系的构建提供参考.
The"milk"industry is one of the"six special"key industries in Ningxia.With the continuous expansion of dairy farming scale,the positive rate of cow diseases remains high.It is necessary to build a large-scale biological safety prevention and control system for dairy farms.This study used ELISA method to detect several common viral diseases in 100 randomly collected blood samples from a large-scale dairy farm in Ningxia.At the same time,samples of feces and environmental swabs from different cowsheds were collected for PCR detection.The results showed that the cows in the farm were infected with bovine viral diarrhea virus(BVDV),bovine coronavirus(BCoV),bovine rotavirus(BRV),and bovine infectious rhinotracheitis virus(IBRV),and these four viruses have long existed in different herd environments.The results of this study lay the foundation for the construction of an important disease risk monitoring system for large-scale dairy farms.
高艳宇;杨登虎;李知新;王晓亮;申华;王少宇;李继东;龚振兴
宁夏大学动物科技学院,银川 750021宁夏大学动物科技学院,银川 750021||陕西铜川王益区动物疫病预防控制中心,铜川 727000宁夏回族自治区动物疾病预防控制中心,银川 750011宁夏回族自治区动物疾病预防控制中心,银川 750011宁夏农垦贺兰山奶业有限公司,银川 750000宁夏农垦贺兰山奶业有限公司,银川 750000宁夏大学动物科技学院,银川 750021宁夏大学动物科技学院,银川 750021
农业科技
规模化奶牛场牛舍环绕病毒检测分析
Large scale dairy farmsCowshed environmentVirus detectionRisk analysis
《中国奶牛》 2026 (4)
24-29,6
国家自然科学基金项目(32460891)宁夏回族自治区重点研发计划重点项目(2024BBF02027)宁夏回族自治区现代农业产业技术体系建设专项(2025)中国科学院"西部之光"人才培养计划项目(2021).
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