内蒙古绒山羊皮肤毛囊退行相关蛋白质组学研究OA

The regression of secondary hair follicles is an essential process of follicular cell apoptosis.This study inves-tigated the protein expression related to this process at the proteomics level,laying the foundation for preliminarily re-vealing the molecular regulatory mechanism of the apoptosis system in secondary hair follicles of Inner Mongolia cash-mere goat skin.This study employed SWATH technology to compare skin samples of Inner Mongolia cashmere goat from September(anagen)and January(catagen),screening for differentially expressed proteins during the regression of secondary hair follicles and conducting bioinformatics analysis of these proteins.The results showed that under a 1％FDR(false discovery rate)condition,1 792 proteins were detected in catagen,while 2 199 proteins were detected in anagen.A total of 164 proteins were specifically expressed in catagen.Using a P value<0.05 and FC(fold change)>2 or FC<0.5 as thresholds,504 differentially expressed proteins were identified between catagen and anagen of Inner Mon-golia cashmere goat skin hair follicles,among which 250 were upregulated and 254 were downregulated in catagen.The specifically expressed proteins in catagen were primarily involved in biological processes(BP)such as the oxidation-reduction process;in cellular components(CC),they were mainly enriched in extracellular exosomes;and in molecular functions(MF),they were primarily associated with functions such as poly(A)RNA binding.The differen-tially expressed proteins were mainly involved in BP functions such as translation;in CC,they were predominantly en-riched in extracellular exosomes;and in MF,they participated in poly(A)RNA binding.KEGG enrichment analysis in-dicated that both the specifically expressed protein and the differentially expressed protein in catagen were mainly en-riched in metabolic pathways.Additionally,proteins upregulated in catagen and enriched in the TGF-beta signaling pathway were included PPP2CB,THBS1,Rhoa,TGFB1,Skp1,etc.,while the downregulated protein was DCN.A total of 28 hub proteins were identified from the PPI network,16 of which were ribosomal proteins,accounting for 57％.In summary,the TGF-beta signaling pathway and ribosomal proteins play key regulatory roles in hair follicle catagen.