蒙古黄芪环阿屯烷型三萜类化合物及其肾损伤保护作用研究OA
Cycloartane triterpenoids from Astragalus membranaceus var.mongholicus and their renal protection against injury
目的 研究蒙古黄芪Astragalus membranaceus var.mongholicus 环阿屯烷型三萜类化学成分及其顺铂所致肾损伤保护活性.方法 采用硅胶、Sephadex LH-20、ODS 等多种色谱分离技术对蒙古黄芪水提物的非多糖组分进行分离纯化,根据其理化性质和光谱、波谱数据鉴定其结构;并采用顺铂诱导人肾皮质近曲小管上皮细胞(HK-2 细胞)损伤模型,评价了其肾保护活性.结果 从蒙古黄芪中共分离得到 4 个环阿屯烷型三萜,分别鉴定为 12-carboxy-20,24,25-trihydroxy-3-(β-D-xylopyranosyloxy)-6-(β-D-glucopyranosyloxy)-7(8)-ene-9,10-secocycloartane(1)、黄芪皂苷Ⅱ(2)、黄芪甲苷(3)、黄芪皂苷Ⅰ(4).体外活性评价结果显示,化合物 2、3 能够显著改善顺铂所造成的细胞活力下降(P<0.05).结论 化合物1 为新的高环阿屯烷型三萜,命名为高环黄芪皂苷 A,化合物 2、3 具有肾损伤保护作用.
Objective To study cycloartane triterpenoids from Astragalus membranaceus var.mongholicus and their protective effects against cisplatin-induced renal injury.Methods The constituents were isolated from non-polysaccharide fractions of the aqueous extract of Astragalus membranaceus var.mongholicus by employing multiple chromatographic techniques including silica gel,Sephadex LH-20 and ODS.Their structures were identified based on the physicochemical properties as well as spectroscopic and spectrometric data.Their protective activities against renal injury were evaluated by detecting the cell viability of human renal proximal tubular epithelial(HK-2)cells injured by cisplatin.Results Four cycloartane triterpenoids were isolated from Astragalus membranaceus var.mongholicus and identified as 12-carboxy-20,24,25-trihydroxy-3-(β-D-xylopyranosyloxy)-6-(β-D-glucopyra-nosyloxy)-7(8)-ene-9,10-secocycloartane(1),astragaloside II(2),astragaloside Ⅳ(3)and astragaloside Ⅰ(4).Additionally,compounds 2 and 3 could significantly improve the decreased cell viability induced by cisplatin(P<0.05).Conclusion Compound 1 is a novel homocycloartane triterpenoid named homoastragaloside A,and compounds 2 and 3 exhibit protective effects against renal injury.
张一鑫;范春雨;郭慧;马建彤;丁丽琴
天津中医药大学中医药研究院,天津 301617||天津市中药功效物质重点实验室,天津 301617||现代中药创制全国重点实验室,天津 301617天津中医药大学中药学院,天津 301617||天津市中药功效物质重点实验室,天津 301617||现代中药创制全国重点实验室,天津 301617天津中医药大学中医药研究院,天津 301617||天津市中药功效物质重点实验室,天津 301617||现代中药创制全国重点实验室,天津 301617天津中医药大学中药学院,天津 301617||天津市中药功效物质重点实验室,天津 301617||现代中药创制全国重点实验室,天津 301617天津中医药大学中医药研究院,天津 301617||天津市中药功效物质重点实验室,天津 301617||现代中药创制全国重点实验室,天津 301617
医药卫生
蒙古黄芪皂苷环阿屯烷高环黄芪皂苷A肾保护活性
Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiaosaponinscycloartanehomoastragaloside Arenal protective activity
《中草药》 2026 (10)
3693-3699,7
国家重点研发计划项目(2022YFC3501601)
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