甜瓜TIFY基因家族成员鉴定及低温胁迫响应表达分析OA

To investigate the role of the TIFY gene family in the low temperature stress response of melon(Cucumis melo L.),genome-wide identification of TIFY family members was performed using bioinformatics approaches.Tissue-specific expression patterns and low temperature stress responsive profiles were further analyzed through transcriptome sequencing and quantitative reverse transcription polymerase chain reaction technology.The results demonstrated that a total of 15 TIFY family genes were identified in melon,classified into four subfamilies:JAZ,TIFY,ZIM,and PPD,and distributed across nine chromosomes.Tissue-specific expression analysis revealed that CmTIFY genes were expressed in various tissues.Among them,CmTIFY5A,CmTIFY5B,CmTIFY5C,CmTIFY5D and CmTIFY9B exhibited higher expression levels in flowers compared to other tissues.With fruit ripening,the gene expression level of CmTIFY5B gradually increased,whereas that of CmTIFY6B gradually decreased.CmTIFY5B and CmTIFY6B may be involved in the regulation of melon fruit ripening and quality formation.The expression levels of most CmTIFY genes were up regulated under low temperature stress,among which CmTIFY5B and CmTIFY5C were most significantly up regulated under low temperature.