西瓜卵形家族蛋白编码基因ClOFP16的克隆及功能研究OA

Ovate family proteins(OFP)constitute a class of plant-specific transcription factors implicated in intracellular signal transduction and biosynthetic processes in plants.Utilizing the watermelon cultivar'TN07011',the coding sequence(CDS)of ClOFP16 were isolated and performed functional characterization.Results demonstrate that ClOFP16 comprises 1 020 base pairs,encoding a protein of 339 amino acids.The encoded protein harbors an N-terminal DNA-binding domain and a C-terminal conserved Ovate domain.Subcellular localization assays confirmed the nuclear localization of ClOFP16.RT-qPCR analysis demonstrated that ClOFP16 transcript abundance was significantly upregulated under salt and 4℃cold stress,but downregulated under drought stress.Arabidopsis thaliana overexpressing ClOFP16 exhibited reduced seed longitudinal diameter in T3 generation.Under salt and low-temperature stress conditions,T3 generation plants exhibited a significant upregulation of ClOFP16 expression.Concurrently,the activities of the antioxidant enzymes SOD,POD,and CAT were markedly increased.Furthermore,total chlorophyll content and soluble protein content were significantly elevated,while the accumulation of malondialdehyde(MDA)was reduced.Collectively,these findings implicate ClOFP16 in pivotal roles governing plant growth and development,as well as in mediating adaptive responses to abiotic stress.