褐藻寡糖诱导CsWRKY46调控黄瓜幼苗响应冷胁迫及转录组分析OA

This paper aims to explore the molecular mechanism of CsWRKY46 gene induced by alginate oligosaccharides in cucumber response to cold stress.In this study,cucumber CU2(wild type)and CsWRKY46 gene knockout type were used as materials,and 0.8％alginate oligosaccharides was sprayed before 4℃cold stress treatment.The effects of alginate oligosaccharides on antioxidant enzyme activity,malondialdehyde content and osmotic adjustment substance content of cucumber seedlings were analyzed,and transcriptome sequencing analysis was carried out.The results showed that the pretreatment of alginate oligosaccharide significantly improved the antioxidant capacity and cold tolerance of cucumber seedlings under cold stress.Transcriptome sequencing results showed that the differentially expressed genes induced by alginate oligosaccharides were mainly enriched in plant hormone signal transduction and protein processing,among which the gene expression of abscisic acid and brassinolide signaling pathways was significantly up-regulated.These results suggest that alginate oligosaccharides may enhance the tolerance of cucumber to cold stress by regulating CsWRKY46 gene and its downstream signaling pathway.