丛枝菌根真菌与嗜松篮状菌LZ1协同缓解苹果连作障碍的机理研究OA

This study aimed to develop a green and sustainable biocontrol strategy for apple replant disease(ARD)by exploring the alleviating effect of combined application of an arbuscular mycorrhizal fungi(Paraglomus sp.SW1)and an antagonistic Talaromyces pinophilus LZ1 on ARD.The results showed that the LZ1 strain isolated from the rhizosphere of chestnut not only significantly inhibited the growth of the main pathogenic Fusarium species causing ARD,but also increased the mycorrhizal colonization rate in apple seedlings grown in replant soil.Validation through greenhouse,pot,and field experiments revealed that the combined application of AMF and LZ1 significantly enhanced the biomass and mycorrhizal colonization in apple plants under replant soil.Compared with the replant control soil treatment without any microbial inoculants(CK-1),plant height,ground diameter,fresh weight,dry weight,and mycorrhizal colonization rate increased by 85.43%,88.20%,144.47%,145.40%,and 62.94%,respectively.The co-inoculation of AMF and LZ1 also significantly increased soil spore density and easily extractable glomalin-related soil protein(EE-GRSP)content,which were 59.87%and 46.98%higher than those in CK-1,respectively.Furthermore,the co-inoculation improved soil enzyme activities and optimized the soil microbial community structure.In summary,the synergistic application of AMF and LZ1 can provide a sustainable and eco-friendly strategy for alleviating ARD.