枳丛枝菌根特异性脂质分配模式对干旱胁迫的响应OA
Arbuscular Mycorrhizal Fungi-Specific Lipid Allocation Patterns in Citrus Mycorrhizae in Response to Drought Stress
以枳[Poncirus trifoliata(L.)Raf.]为植物材料,丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)Rhizophagus irregularis DAOM197198 为供试菌种,在正常水分(土壤含水量 18%)或干旱胁迫(土壤含水量 10%)下接种或不接种 AMF,通过测定根系和土壤中脂质含量、相关基因表达水平等,以揭示柑橘丛枝菌根特异性脂质分配模式对干旱胁迫的响应.结果表明,AMF 显著促进植株生长,干旱 1 周(低强度干旱)和 4 周(高强度干旱)后生物量分别提高 49.09%和 29.76%;干旱显著抑制 AMF 侵染,显著降低AMF的特异性脂肪酸(C16∶1ω5)含量.干旱1周和4周后,根内特异性磷脂脂肪酸(PLFA-C16∶1ω5)含量分别下降 18.59%和 61.52%,而中性脂脂肪酸(NLFA-C16∶1ω5)含量分别下降 54.08%和 46.05%,表明高强度胁迫对 PLFA-C16∶1ω5 的影响远大于低强度胁迫;正常水分下,NLFA-C16∶1ω5 主要富集于根内,干旱促使其向根外分配,而 PLFA-C16∶1ω5 则主要分布于根外,干旱进一步提高其比例.此外,特异性脂质合成与转运相关基因(PtFatM、PtRAM2、PtSTR、PtSTR2)、磷转运基因 PtPT4 和糖转运基因 PtSWEET2 的表达均受干旱抑制.综上所述,考虑到 PLFA-C16∶1ω5 和 NLFA-C16∶1ω5 分别与丛枝(菌丝)和孢子(泡囊)的紧密相关性,可以认为 AMF 通过调节脂质在功能(PLFA vs.NLFA)与空间(根内 vs.根外)上的差异化分配机制来响应干旱胁迫.
With trifoliate orange[Poncirus trifoliata(L.)Raf.]as plant materials inoculated with arbuscular mycorrhizal fungus(AMF)Rhizophagus irregularis DAOM197198 or not,this study investigated the allocation patterns of arbuscular mycorrhizal fungi(AMF)-specific lipids in response to drought stress under well watered(soil water content 18%)or drought stressed(soil water content 10%)conditions,in which the lipid contents in roots and soils and the expression levels of related genes were determined.Results showed that AMF significantly promoted plant growth,with the biomass increasing by 49.09%and 29.76%respectively after 1(weak stress)and 4(strong stress)weeks of drought.Drought significantly inhibited AMF colonization,and significantly reduced the AMF-specific lipid(C16∶1ω5)content as well.The specific phospholipid fatty acid(PLFA-C16∶1ω5)contents decreased by 18.59%and 61.52%respectively after 1(weak stress)and 4(strong stress)weeks of drought,while the specific neutral lipid fatty acid(NLFA-C16∶1ω5)contents decreased by 54.08%and 46.05%,indicating the greater effect of strong stress on PLFA-C16∶1ω5 than that of weak stress.Under well watered condition,NLFA-C16∶1ω5 mainly distributed intraradically with drought promoting it to distribute outwards in soils,while PLFA-C16∶1ω5 mainly distributed extraradically with drought further increasing its proportion in soils.Moreover,drought inhibited the expression of genes related to specific lipid synthesis and transport(PtFatM,PtRAM2,PtSTR,PtSTR2),P transporter PtPT4 and sugar transporter PtSWEET2.In conclusion,considering the close relations of PLFA-C16∶1ω5 with arbuscule(hyphae),and NLFA-C16∶1ω5 with spores(vesicles),it is acceptable that AMF respond to drought stress by differentially allocating lipids based on function(PLFA vs.NLFA)and spatial distribution(intraradical vs.extraradical).
张微;尹喜龙;冯曾威;刘晓迪;周杨;朱红惠;姚青
华南农业大学园艺学院,农业农村部华南地区园艺作物生物学与种质创制重点实验室,广东省微生物信号与病害防治重点实验室,广东省荔枝工程中心,广州 510642华南农业大学园艺学院,农业农村部华南地区园艺作物生物学与种质创制重点实验室,广东省微生物信号与病害防治重点实验室,广东省荔枝工程中心,广州 510642广东省科学院微生物研究所,华南应用微生物国家重点实验室,农业农村部农业微生物组学与精准应用重点实验室,农业农村部农业微生物组学重点实验室,广州 510075广东省科学院微生物研究所,华南应用微生物国家重点实验室,农业农村部农业微生物组学与精准应用重点实验室,农业农村部农业微生物组学重点实验室,广州 510075广东省科学院微生物研究所,华南应用微生物国家重点实验室,农业农村部农业微生物组学与精准应用重点实验室,农业农村部农业微生物组学重点实验室,广州 510075广东省科学院微生物研究所,华南应用微生物国家重点实验室,农业农村部农业微生物组学与精准应用重点实验室,农业农村部农业微生物组学重点实验室,广州 510075华南农业大学园艺学院,农业农村部华南地区园艺作物生物学与种质创制重点实验室,广东省微生物信号与病害防治重点实验室,广东省荔枝工程中心,广州 510642
农业科技
枳干旱胁迫丛枝菌根真菌AMF特异性脂肪酸C16∶1ω5脂质分配
Poncirus trifoliatadrought stressarbuscular mycorrhizal fungiAMF-specific fatty acidsC16∶1ω5lipid allocation
《园艺学报》 2026 (4)
1013-1024,12
国家自然科学基金项目(32570110,32200087,32570127)广东省农业农村厅"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2024KJ19)
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