基于"标准汤剂"理念的绿豆质量评价:多维度鉴别与双指标定量OA

Objective To establish a new comprehensive quality evaluation system for mung bean based on the quality transfer concept of the"standard decoction",using water-soluble extractives as the core index and integrating multi-dimensional identification with quantitative determination of key components.Methods Eighteen batches of mung bean samples were collected from various regions across China.Microscopic identification was performed to observe tissue and powder characteristics.A specific thin-layer chromatography(TLC)method was established.According to methods described in the general chapters of the Chinese Pharmacopoeia(2025 Edition,Volume IV),the content of moisture,total ash,and water-soluble hot-extraction extractives were determined.High-performance liquid chromatography(HPLC)was used to quantify the content of vitexin and isovitexin in the extractives,and correlation analysis was carried out on the indicators.Results The content of vitexin ranged from 0.44 to 0.80 mg/g,and that of isovitexin from 0.37 to 0.71 mg/g.A statistically significant positive correlation was found between the extractive content and the total amount of the two flavonoid glycosides(correlation coefficient r=0.469,P<0.05).Conclusion The multi-dimensional evaluation system established in this study can comprehensively reflect the quality of mung bean,providing a basis for clinical application and the improvement of quality standards.