肺腺癌组织中DTX2、PHF12表达与临床病理特征及预后的关系OA

Objective To study the expression of Deltex E3 ubiquitin ligase 2(DTX2)and PHD finger protein 12(PHF12)in lung adenocarcinoma and their relationship with clinicopathological features and prognosis.Methods Clinical da-ta of 98 patients with lung adenocarcinoma admitted to the Department of Oncology of Tongchuan People's Hospital from February 2020 to February 2022 were selected.R language was used to analyze the expression of DTX2 mRNA and PHF12 mRNA in the TCGA database.The expression of DTX2 mRNA/protein and PHF12 mRNA/protein was verified by quantitative real-time PCR and immunohistochemistry.Kaplan-Meier survival analysis and Cox regression analysis were used to evaluate the impact of DTX2 and PHF12 expression on the prognosis of lung adenocarcinoma.Results TCGA database analysis showed that the expression levels of DTX2 mRNA and PHF12 mRNA in lung adenocarcinoma tissues were signifi-cantly higher than those in normal lung tissues(t=16.909,14.600,both P＜0.001).The expression of DTX2 mRNA was posi-tively correlated with that of PHF12 mRNA in lung adenocarcinoma tissues(r=0.566,P＜0.001).qPCR results showed that the expression levels of DTX2 mRNA and PHF12 mRNA in lung adenocarcinoma tissues were(2.62±0.48)and(3.12±0.51),re-spectively,which were significantly higher than those in adjacent normal tissues(0.94±0.25,1.04±0.27)(t=30.730,35.682,both P＜0.001).Immunohistochemistry results showed that the positive rates of DTX2 and PHF12 in lung adenocarcinoma tis-sues were 63.27％(62/98)and 61.22％(60/98),respectively,which were significantly higher than those in adjacent normal tis-sues(6.12％[6/98]and 5.10％[5/98])(x2=70.618,69.630,both P＜0.001).The positive rates of DTX2 and PHF12 protein were significantly higher in patients with TNM stage Ⅲ A and lymph node metastasis than in those with TNM stage Ⅰ-Ⅱand without lymph node metastasis(x2/P=15.941/＜0.001,19.542/＜0.001;14.930/＜0.001,18.382/＜0.001).The 3-year progres-sion-free survival rates of patients in the DTX2-positive and PHF12-positive groups were 33.87％(21/62)and 33.33％(20/60),respectively,which were significantly lower than those in the negative groups(72.22％[26/36]and 71.05％[27/38])(Log-rank x2=15.440,21.390,both P＜0.001).TNM stage Ⅲ A,lymph node metastasis,DTX2 positivity,and PHF12 positivity were independent risk factors affecting the prognosis of lung adenocarcinoma[HR(95％CI)=1.330(1.091-1.621),1.297(1.070-1.572),1.354(1.041-1.761),1.288(1.040-1.595)].Conclusion The expression of DTX2 and PHF12 is upregulated in lung adenocarcinoma and is associated with TNM stage and lymph node metastasis.These proteins may serve as novel markers for prognostic evaluation in non-small cell lung cancer.