非小细胞肺癌组织FIGNL1、UCHL3表达与同源重组修复基因的相关性及其临床预后意义OA

Objective To investigate the expression of fidgetin like factor 1(FIGNL1)and ubiquitin carboxy-termi-nal hydrolase L3(UCHL3)in non-small cell lung cancer(NSCLC)tissues,and to analyze their correlation with homologous recombination repair genes and prognostic significance.Methods A total of 148 NSCLC patients from the Department of Cardiothoracic Surgery,Nanjing Hospital Affiliated to Nanjing Medical University(Nanjing First Hospital)were collected from June 2018 to June 2021.TCGA database data and qPCR experiments were used to detect the expression of FIGNL1 mRNA,UCHL3 mRNA,breast cancer susceptibility gene 1(BRCA1)mRNA,and excision repair cross-complementing gene 1(ERCC1)mRNA.Pearson correlation analysis was used for correlation assessment.FIGNL1 and UCHL3 proteins were detec-ted by immunohistochemistry.Kaplan-Meier curves and multivariate Cox regression analysis were used to investigate the im-pact of FIGNL1 and UCHL3 protein expression on prognosis.Results TCGA database and qPCR experiments showed that the expression levels of FIGNL1 mRNA,UCHL3 mRNA,BRCA1 mRNA,and ERCC1 mRNA in cancer tissues were signifi-cantly higher than those in adjacent normal tissues(t/P=11.630/＜0.001,18.582/＜0.001,10.721/＜0.001,6.716/＜0.001;44.234/＜0.001,36.435/＜0.001,49.040/＜0.001,38.602/＜0.001).The expression levels of FIGNL1 mRNA,UCHL3 mRNA,BRCA1 mRNA,and ERCC1 mRNA in NSCLC tissues were positively correlated with each other(r=0.661,0.589;0.710,0.632,all P＜0.001).The positive rates of FIGNL1 and UCHL3 in NSCLC cancer tissues were 72.97％(108/148)and 68.92％(102/148),respectively,which were significantly higher than those in adjacent normal tissues(9.46％[15/148]and 8.11％[12/148])(x2=120.31,115.56,both P＜0.001).The positive rates of FIGNL1 and UCHL3 in cancer tissues were significantly higher in patients with TNM stage Ⅲ A and lymph node metastasis than in those with stage Ⅰ-Ⅱ and without lymph node metastasis(x2/P=19.592/＜0.001,7.801/0.005,14.520/＜0.001,6.891/0.009).The mean 3-year survival time of the FIGNL1-positive group was(28.97±0.95)months,which was significantly lower than that of the negative group(34.11±0.75)months(Log-rank x2=13.627,P＜0.001).The mean 3-year survival time of the UCHL3-positive group was(28.72±0.96)months,which was significantly lower than that of the negative group(34.67±0.51)months(Log-rank x2=11.342,P＜0.001).FIGNL1 positivity,UCHL3 positivity,TNM stage Ⅲ A,and lymph node metastasis were independent risk factors affecting the prognosis of NSCLC patients[HR(95％CI)=1.330(1.080-1.637),1.298(1.082-1.559),1.189(1.020-1.385),1.240(1.001-1.535)].Con-clusion The upregulation of FIGNL1 and UCHL3 expression in NSCLC is associated with the expression of homologous re-combination repair genes.These proteins may serve as novel tumor markers for prognostic evaluation of NSCLC.