黄精多糖对高糖诱导的晶状体上皮细胞内质网应激及凋亡的影响OA

Objective To investigate the effects of Polygonatum sibiricum polysaccharide(PSP)on endoplasmic re-ticulum stress(ERS)and apoptosis of lens epithelial cells(LECs)induced by high glucose(HG).Methods SRA01/04 cells were divided into the Control group,HG group,and HG+PSP group.Cell viability was measured by the Cell Counting Kit-8(CCK-8)assay to determine the optimal concentration of PSP.An in vitro rat lens culture model was established to observe the effect of PSP on lens opacity.The expression levels of ERS-related proteins,including phosphorylated protein kinase-like endoplasmic reticulum kinase(p-PERK),protein kinase-like endoplasmic reticulum kinase(PERK),transcrip-tion factor C/EBP homologous protein(CHOP),and glucose-regulated protein 78(GRP78),as well as apoptosis-related proteins,including B-cell lymphoma/leukemia-2(Bcl-2)and Bcl-2-associated X protein(Bax)were detected by Western blot.The expression and distribution of GRP78 were measured by immunofluorescence staining,and cell apoptosis was quantified by TUNEL assay.Results CCK-8 assay showed that compared with the Control group,cell viability in the HG group significantly decreased(P＜0.001).Compared with the HG group,cell viability increased to varying degrees after in-tervention with different concentrations of PSP,with the most significant increase observed at a PSP concentration of 25.00μmol·L-1(P＜0.01).In the in vitro lens culture,the percentage of lens opacity in the HG group was significantly higher than that in the Control group(P＜0.001),while it decreased significantly in the HG+PSP group compared with the HG group(P＜0.01).Western blot results revealed that compared with the Control group,the expression levels of p-PERK/PERK,GRP78,and CHOP proteins were up-regulated,while the Bcl-2/Bax ratio was down-regulated in the HG group(all P＜0.05).Compared with the HG group,the expression levels of the aforementioned ERS-related proteins significantly de-creased,while the Bcl-2/Bax ratio significantly increased in the HG+PSP group(all P＜0.05).Immunofluorescence stai-ning showed that the fluorescence intensity of GRP78 in the HG group was significantly higher than that in the Control group(P＜0.01),while it was significantly reduced in the HG+PSP group compared with the HG group(P＜0.01).TUNEL assay demonstrated that the apoptosis level in the HG group increased compared with the Control group,which was effectively at-tenuated by PSP treatment(all P＜0.05).Conclusion PSP can delay the progression of lens opacity by inhibiting HG-in-duced ERS and apoptosis in LECs.