基于网络药理学探讨安乐阴方外治外阴阴道假丝酵母菌病的作用机制研究OA

Objective To investigate the mechanism of action of Anle Yin Formula in the external treatment of vulvovagi-nal candidiasis(VVC)using network pharmacology.Methods The main chemical constituents and their potential targets of Anle Yin Formula were identified through databases including TCMSP,PubChem,Swiss ADME,and Swiss Target Predic-tion.VVC-related genes were retrieved from the GeneCards and OMIM databases,and the intersection between the two sets was determined.A protein-protein interaction(PPI)network was constructed using the STRING database.A network dia-gram of drug compound-traditional Chinese medicine-component-target was created through Cytoscape 3.10.3.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis were conducted via the Weishengxin cloud platforman—an online tool for bioinformatics analysis and visualization.Molecular docking validation was performed using the CB-Dock2 website.Results Through network pharmacology analysis,94 active chemical components in Anle Yin Formula,954 drug targets,and 2924 VVC-related target genes were identified,with 429 overlapping targets between the drug and the disease.Five core targets—AKT1,GAPDH,TP53,TNF,and STAT3—were ultimately determined.This sug-gested that Anle Yin Formula might reduce cell apoptosis,regulate autophagy and cell adhesion,and affect cell proliferation and differentiation through signaling pathways such as proteoglycan,lipid,and atherosclerosis in cancer(including pathways related to proteoglycan,lipid,and atherosclerosis in cancer),and thereby exerting anti-inflammatory effects to treat VVC.Molecular docking results indicated that Diosmetin,Skimmianin and Xanthoxylin N in Anle Yin Formula exhibited certain af-finity for AKT1,GAPDH,and TP53.Conclusion Anle Yin Formula may exert therapeutic effects on VVC through multi-ple active ingredients,multiple targets,and multiple signaling pathways.