Hsa_circ_0000467在肝细胞癌中的临床意义及生信分析OA

Objective To mine differentially expressed circular RNAs(circRNAs)in hepatocellular carcinoma(HCC)based on public databases,screen core regulatory molecules,predict their potential mechanisms of action,so as to provide novel targets for the diagnosis and treatment of HCC.Methods CircRNA expression profile datasets GSE121714 and GSE242797,which included HCC tissues and adjacent normal tissues,were retrieved from the gene expression omnibus(GEO)database.Differential expression analysis was performed on the above data using the limma package in R language to identify significantly differentially expressed circRNAs.The circbank and circinteractome databases were used to predict mi-croRNAs(miRNAs)that might bind to these differentially expressed circRNAs.Potential downstream target genes of these miRNAs were determined by integrating the prediction results from the TargetScan,miRDB and miRTarBase databases.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)functional enrichment analyses based on single-li-brary genes were conducted to provide core clues for molecular mechanism research.Finally,quantitative real-time polymer-ase chain reaction(qRT-PCR)was used to verify the differential expression of the core circRNA in HCC cell lines(MH-CC97H,Huh7)and normal liver cell line(LO2).Results Differentially expressed circRNAs were screened by taking the intersection of the two datasets,including 16 up-regulated and 28 down-regulated circRNAs.A competing endogenous RNA(ceRNA)network was constructed with hsa_circ_0000467 as the core molecule.The qRT-PCR verification results showed that the expression level of hsa_circ_0000467 in HCC cell lines was significantly higher than that in the normal liver cell line(P<0.05).Conclusion Hsa_circ_0000467 may promote the occurrence and development of HCC through the circRNA/miRNA/mRNA regulatory axis,and is expected to serve as a potential molecular marker for prognostic evaluation of HCC.