基于网络药理学和分子对接探讨人参皂苷Rg1治疗卵巢早衰的作用机制OA

Objective To investigate the signaling pathways of ginsenoside Rg1 in the treatment of premature ovarian failure(POF)using network pharmacology and molecular docking methods,and to predict its possible mechanisms of action.Methods The target genes of ginsenoside Rg1 and POF were obtained from the CTD,GeneCards,HERB,and OMIM data-bases.The overlapping genes between the two were screened using the Venn online tool,and then entered into the STRING database and Cytoscape 3.10.0 for analysis to construct a protein-protein interaction(PPI)network.Enrichment analysis was conducted by the DAVID database.Finally,molecular docking was performed using AutoDock vina,and the results were visualized using PyMol.Results A total of 155 target genes of ginsenoside Rg1 and 949 disease target genes of POF were screened.71 overlapping target genes between the drug and the disease were identified and they were imported into the STRING database to generate a"drug-disease"protein interaction network.Potential core targets,including TP53,AKT1,CTNNB1,ESR1,PIK3CA,TNF,and IL-6,were explored.Enrichment analysis was conducted through the DAVID data-base,and the results showed that the main biological processes included positive regulation of gene expression,negative reg-ulation of gene expression,and response to external stimuli,etc.The main signaling pathways included p53 signaling path-way,PI3K/AKT signaling pathway,and Wnt/β-catenin signaling pathway,etc.Conclusion Ginsenoside Rg1 may exert therapeutic effects on premature ovarian failure through multiple pathways and multiple targets,which can provide a theoreti-cal basis for further experimental research and clinical application.