miR-548b靶向调控APC2对结直肠癌进展的影响OA

Objective It is to investigate the expression characteristics of miR-548b in colorectal cancer(CRC)and its effect on the invasion and proliferation of CRC cells via targeting regulation of adenomatous polyposis coli protein 2(APC2).Methods ①Paired tumour and adjacent non-cancerous tissue samples were collected from 60 CRC patients un-dergoing surgical resection and pathologically confirmed at the Second Hospital of Hebei Medical University from January 2021 to December 2022,the expression of miR-548b in tissue samples was detected by qRT-PCR,and the clinical charac-teristics of patients with high and low expressions of miR-548b were compared.②The CRC cell lines SW480,HT29,SW620,HCT116,LOVO and normal intestinal epithelial cells FHC were cultured,the expression of miR-548b in the cells was detected by qRT-PCR,The SW620 cells with high expression of miR-548b were screened for the subsequent experi-ments.A miR-548b mimic group and a normal control(NC)group were set up in the experiment,the effects of miR-548b on the invasion and proliferation of SW620 cells were evaluated by Transwell and MTT assays.The miR-548b target genes were predicted by Bioinformatics analysis,and the targeting regulatory relationship between miR-548b and APC2 was vali-dated by luciferase reporter assay and Western blot analysis.Results The relative expression of miR-548b in tumor tissues of CRC patients was significantly higher than that in adjacent normal tissues(P<0.05),furthermore,the proportions of patients with lymph node metastasis and those with Dukes'stage C or D were significantly higher in the miR-548b high-ex-pression group than those in the miR-548b low-expression group(all P<0.05 for).The number of invading cells in the miR-548b mimic group was significantly higher than that in the NC group(P<0.05),and the cell survival rates in the miR-548b mimic group after 48 and 72 hours of culture were both significantly higher than those in the NC group(both P<0.05).APC2 was confirmed to be a direct target gene of miR-548b,and the relative expression of APC2 protein in the miR-548b mimic group was significantly lower than that in the NC group(P<0.05).Conclusion MiR-548b is highly ex-pressed in CRC tissue and it can promote the invasion and proliferation of tumor cells via targeting inhibition of APC2,this suggests that it may serve as a potential diagnostic and therapeutic target for CRC.