白果温胆汤对卵清蛋白诱导的支气管哮喘大鼠肺功能及气道炎症的影响OA

Objective It is to observe the effects of Baiguo Wendan Decoction on pulmonary function and airway inflam-mation in rats with bronchial asthma.Methods Sixty male SD rats were divided into blank group,model group,dexametha-sone group,low-dose,medium-dose and high-dose groups of Baiguo Wendan Decoction according to the random number ta-ble,with 10 rats in each group.The rats of all groups except for the blank group were sensitized and stimulated with ovalbumin to establish models of bronchial asthma.After the last nebulization,the respiratory rate and enhanced expiratory pause(Penh value)of the rats were measured by non-invasive whole-body plethysmograph;the serum levels of immuno-globulin E(IgE)and immunoglobulin G1(IgG1)were measured by ELISA;the histopathological features of lung and bronchial tissues in the rats were observed by HE staining;the protein expressions of tumor necrosis factor-α(TNF-α)and interferon α1(IFN-α1)in lung tissue were detected by Western blot,and the mRNA expressions of interleukin(IL)-6,IL-1β,IL-18,and interferon β(IFN-β)in lung tissue were detected by RT-qPCR.Results Compared with the control group,the respiratory rate,Penh values after acetylcholine stimulation at various concentrations,and serum levels of IgE and IgG1 in rats of the model group were all significantly increased(all P<0.05);thickened alveolar walls and intralun-gary bronchial walls,partial alveolar collapse,narrowing of bronchial lumen with marked inflammatory cell infiltration were found in the rats;the relative protein expressions of TNF-α and IFN-α1,as well as the relative mRNA expressions of IL-6,IL-1β,IL-18,and IFN-β in lung tissue were all significantly increased(all P<0.05).Compared with the model group,the respiratory rate,Penh values after acetylcholine stimulation at various concentrations(except for the Penh value after 0 mg/mL acetylcholine stimulation in the low-dose group of Baiguo Wendan Decoction),and serum levels of IgE and IgG1 were all significantly reduced in rats of the medicated groups(all P<0.05);the pathological changes in both lung and bronchial tissues of the rats were significantly alleviated;the relative protein expressions of TNF-α and IFN-α1,as well as the rela-tive mRNA expressions of IL-6,IL-1β,IL-18,and IFN-β in lung tissue were all significantly reduced(all P<0.05).Conclusion Baiguo Wendan Decoction can control airway inflammation and improve lung function of rats with bronchial asthma.