干旱胁迫对蒙古沙冬青种子萌发及幼苗生长的影响OA
Effects of Drought Stress on Seed Germination and Seedling Growth of Ammopiptanthus mongolicus
探究不同干旱胁迫程度对蒙古沙冬青种子萌发及后续幼苗生长与生理的影响,为干旱区植物育种与生态修复提供借鉴.设置质量分数梯度为 0%、2%、4%、6%、8%、10%、12%、14%、16%、18%、20%的 PEG-6000 溶液模拟不同强度干旱胁迫,研究蒙古沙冬青种子发芽情况、幼苗形态指标及生理生化特性变化.结果表明,低质量分数对种子萌发具有促进作用,且幼苗的胚轴更为健壮;随着质量分数升高,种子萌发受到抑制,当质量分数≥12%时,发芽率低于 50%,发芽势低于10%,幼苗的子叶将会无法正常展开,胚根的发育受阻甚至缺失;随胁迫强度提高,脯氨酸含量呈现先升高后降低的趋势,丙二醛含量持续升高,SOD、P OD、CAT 活性整体呈现出先升高后降低的趋势,且对含量的敏感性存在不同程度的差异.综上所述,本试验通过 PEG-6000 模拟干旱胁迫对蒙古沙冬青种子萌发与幼苗形态建立的影响,发现"适度促进、过度抑制"的动态响应规律,并得出 12%PEG-6000 为模拟蒙古沙冬青种子干旱胁迫的适宜质量分数,此时种子达半致死,萌发及幼苗生长生理受到显著抑制.
To clarify the effects of drought stress on seed germination and subsequent seedling growth and physiology of Ammopiptanthus mongolicus,so as to provide reference for plant breeding and ecological restoration in arid areas.PEG-6000 solution with gradient contents(0%,2%,4%,6%,8%,10%,12%,14%,16%,18%,20%)was set to simulate drought stress with different intensities to study the seed ger-mination,seedling morphological indexes and physiological and biochemical characteristics of A.mongoli-cus.The result showed that,Low content promoted seed germination,and seedling hypocotyls were more robust.As content increased,seed germination was inhibited.When the content was≥12%,germination rate was below 50%,germination potential below 10%,seedling cotyledons failed to unfold normally,and radicle development was hindered or even absent.With increasing stress intensity,proline(Pro)content first increased and then decreased,malondialdehyde(MDA)content continued to increase,and activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)generally showed a first increasing and then decreasing trend,with different degrees of concentration sensitivity.PEG-6000 effectively simu-lates the effects of drought stress on seed germination and seedling growth of A.mongolicus,and a dynam-ic response law of"moderate promotion and excessive inhibition"is revealed.The study concludes that 12%PEG-6000 is the suitable content for simulating drought stress on A.mongolicus seeds,at which seeds reach semi-lethality,and germination and seedling growth physiology are significantly inhibited.
邓煜;马正政;王先英;鲁松辉;侯佳
内蒙古农业大学 园艺与植物保护学院,内蒙古 呼和浩特 010018内蒙古农业大学职业技术学院,内蒙古 包头 014109内蒙古农业大学职业技术学院,内蒙古 包头 014109内蒙古农业大学职业技术学院,内蒙古 包头 014109内蒙古农业大学职业技术学院,内蒙古 包头 014109
农业科技
蒙古沙冬青PEG-6000干旱胁迫种子萌发幼苗生长
Ammopiptanthus mongolicusPEG-6000drought stressseed germinationseedling growth
《西北林学院学报》 2026 (3)
38-46,9
内蒙古自治区高等学校碳达峰碳中和研究专项(STZX20222).
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