基于环境DNA技术的海河干流浮游动植物资源调查与分析OA

To investigate the species composition and community structure of zooplankton and phytoplankton in the Haihe River mainstream under different intensities of human activity disturbance,water samples were collected from 12 sites along the Haihe River mainstream.The situation of plankton resources in this area was investigated using environmental DNA(eD-NA)technology combined with 18S rRNA gene highthroughput sequencing and bioinformatic analysis.Meanwhile,the impacts of high,medium,and low levels of anthropogenic disturbance on zooplankton and phytoplankton were analyzed.The results illustrated that:① A total of 56 species belonging to seven phyla were detected among zooplankton,and the top three phyla were Ciliophora,Rotifera,and Arthropoda,among which Ciliophora was absolutely predominant.The dominant zoo-plankton species common across all sampling sites were Orborhabdostyla bromelicola,Tintinnidium fluviatile,and Vorticella fusca,and Sinocalanus sinensis was the dominant species with the highest relative abundance in the entire survey area.② A total of 52 species belonging to four phyla of phytoplankton were detected,and the top three phyla were Bacillariophyta,Chlorophyta,and Cryptophyta.The dominant phytoplankton species common across all sites were Uncultured Cryptomonas,Cyclotella meneghiniana,Cryptomonas curvata,etc.③ In terms of the diversity of the community structure,the differences in the Alpha diversity index and Beta diversity index of zooplankton were not significant(P＞0.05)among the groups with dif-ferent intensities of human activity disturbance,while the Alpha diversity index of phytoplankton was not significant(P＞0.05),but the difference in the Beta diversity index was significant(P＜0.05)among different groups,which meant that phytoplankton was more sensitive than zooplankton when facing anthropogenic disturbance.④ Bacillariophyta was dominant in phytoplankton and the species belonging to Cyanophyta were not detected,and Sinocalanus sinensis was the dominant species in zooplankton with a low diversity and relative abundance of Rotifers,which indicates a good overall water quality condition of the Haihe River mainstresm.