不同激素对甜瓜未授粉子房愈伤组织诱导的影响OA

To systematically investigate the effects of different phytohormones and their combinations on the callus induction from in vitro unpollinated ovary culture of melon,different concentrations of GA3,NAA,KT,2,4-D,6-BA and TDZ in individually and in combined treatments were designed,and different concentrations of coconut milk were incorporated into the medium,the effects of these factors on the callus induction rate and the morphological characteristics of callus were analyzed.The results showed that among the different combinations of GA3,TDZ,NAA,and KT in the orthogonal experiment,the order of their effects on the callus induction was NAA>KT>GA3>TDZ.The optimal concentrations were 0.5 mg·L-1NAA,0.1 mg·L-1KT,0.5 mg·L-1GA3 and 0.06 mg·L-1TDZ re-spectively.The combination of 0.5 mg·L-1NAA and 0.1 mg·L-1 KT had the best effect on callus induction,with an induction rate of 52.6%,producing callus with a dense texture and light green color.In the optimised medium containing MS+0.5 mg·L-1NAA+0.1 mg·L-1KT with the addition of 15%coconut milk,the rate of callus induction was enhanced to 68.1%,and browning rate was reduced to 7.8%.Single hormones TDZ,2,4-D,6-BA was prone to cause browning or abnormal growth of callus.In conclusion,the highly effi-cient medium for callus induction from in vitro unpollinated ovary culture of melon is MS medium supplemented with 0.5 mg·L-1 NAA,0.1 mg·L-1 KT,and 15%coconut milk.