地黄对急性痛风性关节炎的预防作用及其机制研究OA

Objective To study the preventive effect and potential mechanism of Rhizoma Rehmanniae extract against acute gouty arthritis(AGA)in rats.Methods Wistar rats were used to establish an AGA rat model.Then,the ankle joint swelling index was observed,and changes in body weight index,kidney index,and liver index were recorded before and after modeling.Serum levels of uric acid,creatinine,xanthine oxidase activity,and the contents of IL-1β,NLRP3,and TNF-α were measured.HE staining was used to observe pathological changes in the ankle joint tissue.Additionally,UPLC-TSQ-MS was employed to analyze the components of Rhi-zoma Rehmanniae aqueous extract and its ethyl acetate extract paste.Results Compared with the model group,the treatment group dem-onstrated good activity against AGA.The levels of uric acid,creatinine,IL-1β,NLRP3,TNF-α,and the xanthine oxidase activity in serum were significantly reduced.Furthermore,UPLC-TSQ-MS analysis showed 18 components in the aqueous extract paste of Rhizoma Rehmanniae and 14 components in the ethyl acetate extract paste.Conclusion Both the aqueous extract and ethyl acetate extract paste of Rhizoma Rehmanniae have good preventive effects on AGA.The mechanism of the action maybe involve inhibiting the release of inflam-matory factors,reducing uric acid production,suppressing xanthine oxidase activity,and promoting creatinine excretion,thereby exert-ing preventive effects against AGA.