当归补血汤干预斑秃的机制研究OA

Objective To preliminarily investigate the mechanism of Danggui Buxue Decoction(当归补血汤,DGBXD)in treating alo-pecia areata(AA)by examining its effects on serum alkaline phosphatase(ALP),insulin-like growth factor(IGF),fibroblast growth factor-7(FGF-7)levels,and their mRNA expression in skin lesions of AA mouse models.Methods AA mice were induced and ran-domly divided into a model group,low-dose DGBXD(2.5 g/kg),medium-dose DGBXD(5 g/kg),high-dose DGBXD(10 g/kg),and vitamin D group(30 ng/kg),with a normal control group receiving saline.Interventions lasted 45 days.Hair loss and regeneration were observed under light microscopy.Pathological changes were analyzed via HE staining.TUNEL staining quantified follicular cell apopto-sis.Serum ALP,IGF,and FGF-7 levels were measured by ELISA.Real-time qPCR assessed mRNA expression of ALP,IGF,and FGF-7 in skin lesions.Results Compared to the normal group,the model group exhibited severe hair loss,reduced hair follicle density,and vacuolar degeneration.Drug-treated groups,especially high-dose DGBXD and vitamin D,showed hair regrowth,increased fol-licles,and reduced vacuolar changes.The model group had significantly elevated follicular apoptosis rates(P<0.05),decreased serum ALP,IGF,FGF-7 levels,and downregulated mRNA expression(P<0.05).In contrast,medium-and high-dose DGBXD and vitamin D groups demonstrated reduced apoptosis rates(P<0.05)and upregulated ALP,IGF,FGF-7 levels and mRNA expression(P<0.05).Conclusion DGBXD may mitigate alopecia areata by modulating ALP,IGF,and FGF-7 expression and alleviating follicular cell apopto-sis,suggesting a multi-target therapeutic mechanism.