男性不育症患者精浆生化指标、精子DNA碎片率与IVF-ET妊娠结局关系研究OA

Objective:To investigate the relationship between seminal plasma biochemical parameters,sperm DNA fragmentation index and in vitro fertilization and embryo transfer(IVF-ET)pregnancy outcomes in male infer-tility patients.Methods:A total of 88 male infertility patients were enrolled.Routine semen analysis,seminal plasma biochemistry,and sperm DNA fragmentation index were performed.Patients and their spouses underwent IVF-ET assisted reproductive treatment and were followed up until ultrasound examination at 28-30 days after embryo trans-fer,with the observation of gestational sacs serving as the criterion for clinical pregnancy.According to the pregnancy outcomes of the patients' spouses,subjects were divided into the successful pregnancy group(40 cases)and the failed pregnancy group(48 cases).Baseline characteristics of patients and their spouses,routine semen parameters,seminal plasma biochemical indices(seminal plasma citric acid,neutral α-glucosidase,zinc and fructose),and sperm DNA fragmentation index were compared between the two groups.Logistic regression analysis was used to identify influencing factors for IVF-ET pregnancy outcomes in male infertility patients.Spearman correlation analysis was performed to analyze the correlations between seminal plasma biochemical parameters,percentage of morphologically normal sperm,sperm DNA fragmentation index,and IVF-ET pregnancy outcomes in male infertility patients.Results:No statistically significant differences were observed in baseline characteristics between the two groups of patients and their spouses(all P＞0.05).No significant differences were found in semen volume,percentage of progressive motile sperm,sperm concentration,or sperm motility rate between the two groups(all P＞0.05).The successful pregnancy group exhibited higher levels of seminal plasma citric acid,neutral α-glucosidase,zinc,and fructose,as well as a higher percentage of morphologically normal sperm,but a lower sperm DNA fragmentation index compared with the failed pregnancy group(all P＜0.05).Seminal plasma citric acid,neutral α-glucosidase,zinc,fructose,percentage of morphologically normal sperm,and sperm DNA fragmentation rate were identified as independent influencing fac-tors for IVF-ET pregnancy outcomes in male infertility patients(all P＜0.05).Seminal plasma citric acid,neutralα-glucosidase,zinc,fructose,and percentage of morphologically normal sperm were positively correlated with IVF-ET pregnancy outcomes,whereas sperm DNA fragmentation index was negatively correlated with IVF-ET pregnancy outcomes(all P＜0.05).Conclusion:Seminal plasma biochemical parameters and sperm DNA fragmentation index are associated with IVF-ET pregnancy outcomes in male infertility patients and serve as influencing factors for IVF-ET pregnancy outcomes.