油酸对胃癌细胞增殖、迁移、侵袭及运动活性的影响实验研究OA

Objective:To investigate the effects of oleic acid on proliferation,migration,invasion and motility of gastric cancer cells.Methods:Human gastric mucosal epithelial cells(GES-1)and human gastric cancer cell lines(AGS,HGC-27,MKN-45,MKN-28)were treated with different concentrations of oleic acid.Intracellular lipid drop-let formation was detected by oil red O staining.Cell viability and proliferation were evaluated by MTT assay and EdU staining.Wound healing assay and Trans well assay were used to observe cell migration and invasion.Real-time cell movement was monitored by high-content cell imaging.The mRNA and protein expression levels of adipose tri-glyceride lipase(ATGL)were detected by RT-qPCR and Western blot.Results:Compared with GES-1 cells,the lipid droplet formation rate was increased in MKN-45,MKN-28,HGC-27 cells at 24 and 48 hours and AGS cells at 48 hours(all P＜0.05),except for AGS cells at 24 hours.Compared with 0 μmol/L oleic acid,the viability of MKN-45 cells was increased at 50-400 μmol/L oleic acid at 24 and 48 hours(all P＜0.05).Compared with 0 μmol/L oleic acid,there was no significant difference in the viability of HGC-27 cells at 50-200 μmol/L oleic acid at 24 and 48 hours(all P＞0.05),while the viability was decreased at 300 and 400 μmol/L oleic acid at 24 and 48 hours(all P＜0.05).Compared with 0 μmol/L oleic acid,the proliferation rate of MKN-45 and HGC-27 cells was increased at 200 μmol/L oleic acid at 24 and 48 hours(all P＜0.05).Compared with 0 μmol/L oleic acid,the migration rate was increased in MKN-45 cells at 48 hours and HGC-27 cells at 24 and 48 hours treated with 200 μmol/L oleic acid(all P＜0.05),except for MKN-45 cells at 24 hours.Compared with 0 μmol/L oleic acid,the number of migrated cells was increased in MKN-45 cells at 24 and 48 hours and HGC-27 cells at 48 hours treated with 200 μmol/L oleic acid(all P＜0.05),except for HGC-27 cells at 24 hours.Compared with 0 μmol/L oleic acid,the number of invasive cells was increased in MKN-45 cells at 24 and 48 hours and HGC-27 cells at 24 hours treated with 200 μmol/L oleic acid(all P＜0.05),except for HGC-27 cells at 48 hours.Compared with 0 μmol/L oleic acid,the mean squared displacement of MKN-45 and HGC-27 cells treated with 200 μmol/L oleic acid showed an upward trend with prolonged observation time,the real-time movement speed of HGC-27 cells was accelerated(P＜0.05),and the motility of both cell lines was en-hanced.Compared with GES-1 cells,the protein expression of ATGL was downregulated in AGS,HGC-27,MKN-45 and MKN-28 cells,but the differences were not statistically significant(all P＞0.05).Compared with GES-1 cells,the mRNA expression of ATGL was downregulated in AGS,HGC-27,MKN-45 and MKN-28 cells,and the differ-ences were statistically significant in HGC-27 and MKN-28 cells(all P＜0.05).Conclusion:Lipid droplet formation ability is higher in gastric cancer cells than in normal gastric epithelial cells,which may be related to the downregula-tion of ATGL expression.Oleic acid can enhance the proliferation,migration,invasion and motility of gastric cancer cells in a cell subtype-specific manner.