川芎嗪对大鼠异位子宫内膜组织生长的抑制作用及其机制OA

Objective To investigate the inhibitory effect of tetramethylpyrazine(TMP)on the growth of ectopic endometrial tissue in rats with endometriosis,and to explore its underlying mechanism by examining the expressions of vascular endothelial growth factor A(VEGFA)and tumor necrosis factor-alpha(TNF-α).Methods An endometriosis model was established in Sprague-Dawley(SD)rats using autologous endometrial implantation.The successfully modeled rats were randomly divided into model group,low-,medium-,and high-dose TMP groups,with 6 rats in each group.The rats in TMP groups were administered with tetramethylpyrazine phosphate solution(1 mL/d)at concentrations of 2.375,4.75,9.5 mg/mL by gavage for 28 consecutive days,respectively,while the rats in model group received an equal volume of normal saline.After the intervention,the volume of ectopic endometrial tissue was mea-sured,and the volume reduction rate was calculated.Histopathological changes were observed using hematoxylin-eosin(HE)staining.The protein levels of VEGFA and TNF-α in ectopic endometrial tissue were detected by immunohis to chemistry,and those in serum and peritoneal lavage fluid were mearured by enzyme-linked immunosorbent assay(ELISA),respectively.Results Compared with model group,the volume reduction rate of ectopic endometrial tissue in medium-and high-dose TMP groups was significantly increased(P<0.05),and the volume reduction rate dose-dependently increased by TMP(P<0.05).Compared with model group,the expression levels of VEGFA and TNF-α in both ectopic endometrial tissue and serum significantly decreased in high-dose TMP group(P<0.05);how-ever,there was no statistically significant difference in the levels of VEGFA and TNF-α in peritoneal lavage fluid among all groups(P>0.05).Conclusion The medium-and high-dose TMP by gavage can effectively inhibit the growth of ectopic endometrial tissue in rats,accompanied by a decrease in the expression levels of VEGFA and TNF-α in both ectopic endometrial tissue and serum.Its mechanism of action may be associated with the inhibition of angiogenesis and the inflammatory microenvironment.