基于网络药理学与数据挖掘的半夏泻心汤抗胃癌关键靶点及作用机制OA
Key target genes and role mechanism of Banxia Xiexin decoction against gastric cancer based on network pharmacology and data mining
目的 基于网络药理学与数据挖掘探讨半夏泻心汤抗胃癌的核心靶基因及潜在机制.方法 基于网络药理学方法,整合癌症基因组图谱(TCGA)等数据库,筛选半夏泻心汤抗胃癌的潜在靶点;依据介数中心性(BC)、度中心性(DC)及接近中心性(CC)得分确定关键靶基因,进行基因本体论(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析.通过生存分析筛选P<0.05且显著性排名前三的基因作为核心靶基因,分析其与胃癌TNM分期及免疫细胞浸润的关联;采用分子对接验证核心靶基因编码蛋白与对应配体的结合能力.结果 从半夏泻心汤1 115个潜在作用靶基因中,获得与胃癌相关靶基因171个,进一步筛选出E Z H 2、F N 1、S E R P I N E1等13个关键靶基因.KEGG富集显示,这些靶基因主要富集于癌症相关通路、IL-17信号通路及HIF-1信号通路.生存分析明确E Z H 2、F N 1、S E R P I N E1为核心靶基因,三者均与胃癌T分期及免疫细胞浸润密切相关;分子对接证实这3种编码蛋白与对应配体结合能力均较强.结论 经网络药理学与数据挖掘预测,E Z H 2、F N 1、S E R P I N E 1为半夏泻心汤抗胃癌的核心靶基因;其作用机制可能与调控IL-17信号通路、HIF-1信号通路等癌症相关通路有关.上述靶基因在胃癌中表达水平与T分期及免疫细胞浸润程度存在关联,所编码蛋白与半夏泻心汤活性成分具有较强结合能力.
Objective To explore core target genes and potential mechanisms of Banxia Xiexin decoction against gastric cancer(GC)based on network pharmacology and data mining.Methods Potential targets of Banxia Xiexin decoction against gastric cancer were screened based on network pharmacology and The Cancer Genome Atlas(TCGA)database analysis.Key target genes were then screened based on betweenness centrality(BC),degree centrality(DC),and closeness centrality(CC)scores.Subsequently,Gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed.The top three most significant genes(P<0.05)identified by survival analysis were selected as core targets,and their rela-tionships with TNM stages and immune cell infiltration in GC were examined.Finally,the binding of the proteins encoded by the core targets to their corresponding ligands were validated through molecular docking.Results Of 1 115 potential targets of Banxia Xiexin decoction,171 associated with GC were identified,and the 13 key targets were further identified,including EZH2,FN1 and SER-PINE1.KEGG enrichment analysis indicated that these targets primarily participate in cancer-related pathways,as well as IL-17 and HIF-1 signaling pathways.Survival difference analysis demonstrated that EZH2,FN1 and SERPINE1 were core target genes,all of which were significantly correlated with T stage and immune cell infiltration in GC.Molecular docking studies confirmed that the pro-teins encoded by these three core targets exhibited strong binding affinities to their respective ligands.Conclusion EZH2,FN1,and SERPINE1 were identified as core targets of Banxia Xiexin decoction against gastric cancer via network pharmacology and data mining.The therapeutic mechanism may involve the regulation of cancer-related pathways,including IL-17 and HIF-1 signaling.Fur-thermore,the expression of these genes in gastric cancer correlates with T stage and immune infiltration,and their encoded proteins show strong binding to the decoction's active components.
涂泽宇;张淑敏;杨培培;黄逍;滕钰浩;徐媛媛;舒鹏
南京中医药大学附属医院肿瘤内科,南京 210029||南京中医药大学第一临床医学院肿瘤内科南京中医药大学附属医院肿瘤内科,南京 210029||南京中医药大学第一临床医学院肿瘤内科南京中医药大学附属医院肿瘤内科,南京 210029||南京中医药大学第一临床医学院肿瘤内科南京中医药大学附属医院肿瘤内科,南京 210029||南京中医药大学第一临床医学院肿瘤内科南京中医药大学附属医院肿瘤内科,南京 210029||江苏省中医院肿瘤内科南京中医药大学附属医院肿瘤内科,南京 210029||江苏省中医院肿瘤内科南京中医药大学附属医院肿瘤内科,南京 210029||南京中医药大学第一临床医学院肿瘤内科||江苏省中医院肿瘤内科
医药卫生
半夏泻心汤胃癌网络药理学数据挖掘作用机制免疫细胞
Banxia Xiexin decoctiongastric cancernetwork pharmacologydata miningmechanism of actionimmune cells
《山西医科大学学报》 2026 (4)
368-378,11
癌症、心脑血管、呼吸和代谢性疾病防治研究国家科技重大专项(2024ZD0521300)国家自然科学基金项目(82374539)江苏省中医药科技发展计划重点项目(ZD202214)江苏省中医药领军人才培养对象项目(SLJ0327)南京中医药大学胃癌临床专病研究院项目(LCZBYJYZZ2024-001)
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