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A practical guide for characterization of novel CRISPR-Cas systems with Pro-CRISPR factorsOA

A practical guide for characterization of novel CRISPR-Cas systems with Pro-CRISPR factors

Ao Sun;Shu-Lin Jin;Jun-Jie Gogo Liu

Beijing Frontier Research Center for Biological Structure,Beijing Advanced Innovation Center for Structural Biol-ogy,State Key Laboratory of Membrane Biology,Tsinghua-Peking Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing 100084,ChinaBeijing Frontier Research Center for Biological Structure,Beijing Advanced Innovation Center for Structural Biol-ogy,State Key Laboratory of Membrane Biology,Tsinghua-Peking Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing 100084,ChinaBeijing Frontier Research Center for Biological Structure,Beijing Advanced Innovation Center for Structural Biol-ogy,State Key Laboratory of Membrane Biology,Tsinghua-Peking Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing 100084,China

CRISPR-Cas systemGenome editingCas nucleasePro-CRISPRProtein purificationBiochemical prop-erties

CRISPR-Cas systemGenome editingCas nucleasePro-CRISPRProtein purificationBiochemical prop-erties

《生物物理学报(英文)》 2026 (2)

85-99,15

This work was supported by The National Key R&D Program(2022YFF1002801 to JJ.G.Liu),Ministry of Agriculture and Rural Affairs of China(JJ.G.Liu),the New Cornerstone Science Foundation through the XPLORER PRIZE(JJ.G.Liu),The STI2030-Major Projects(2022ZD0213900 to JJ.G.Liu),the National Natural Science Foundation of China(32150018 to JJ.G.Liu and 323B2048 to A.Sun).

10.52601/bpr.2025.240066

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