红阳猕猴桃多糖结构解析及对秀丽隐杆线虫抗衰老作用OA

To investigate the structure of polysaccharides from Actinidia chinensis cv.Hongyang and their antioxidant and anti-aging effects on Caenorhabditis elegans,crude polysaccharides from A.chinensis cv.Hongyang(ACP)were prepared by ultrasound-assisted extraction.ACP were purified sequentially on DEAE-52 cellulose and Sephadex G-100 columns,yielding a homogenous polysaccharide(ACP-3-G).The molecular structure of ACP-3-G was characterized using high performance liquid chromatography(HPLC),high performance gel permeation chromatography(HPGPC),and nuclear magnetic resonance(NMR)spectroscopy.Finally,the effects of different concentrations of ACP-3-G on the lifespan,motility,heat stress resistance,and oxidative stress resistance of C.elegans as a model organism were evaluated to its anti-aging potential.The results showed that the yield of ACP-3-G was(73.42±1.74)%,with higher contents of polysaccharide and uronic acid than ACP.In addition,ACP-3-G was a polysaccharide with a molecular mass of 120.6 kDa.Its monosaccharide composition included mannose(0.367%),rhamnose(2.384%),galacturonic acid(58.859%),glucose(4.501%),galactose(23.942%),and arabinose(9.307%).NMR analysis indicated that the main chain of the polysaccharide was primarily composed of disubstituted sugar residues →1)-α-Araf-(5→,→1)-α-GalpA-(4→,→1)-β-Galp-(4→,and trisubstituted sugar residues →1,3)-β-GalpA-(4→,→1,4)-β-Galp-(6→,and →1,2)-α-GalpA-(4→.Furthermore,ACP-3-G intervention at concentrations of 10,50,100,500,and 1 000 μg/mL significantly improved the lifespan,motility,heat stress resistance,and oxidative stress resistance of C.elegans compared with the control group,with the most pronounced effects observed at 1 000 μg/mL.In conclusion,ACP-3-G is a polysaccharide with antioxidant and anti-aging properties.