发酵饲料对斑点叉尾鮰幼鱼肠道发育、抗氧化能力及免疫功能的影响OA

The experiment aimed to investigate the effects of fermented feed on the intestinal development,antioxidant capacity,and immune function of juvenile Ictalurus punctatus.A total of 216 000 healthy Ictalurus punctatus with an initial weight of(100.79±10.23)g were randomly divided into three groups,with six replicates per group and 12 000 fish per replicate.The control group(group S0)was fed with basic feed throughout the entire process,the interval feeding group(group S1)was fed with fermented feed every five days instead of 20%of basic feed,and the continuous feeding group(group S2)was fed with fermented feed instead of 20%of basic feed throughout the entire process.The pre-test period was 14 days,and the formal test period was 180 days.The results showed that compared with the group S0,the activities of leucine aminopeptidase(LAP)and alkaline phosphatase(AKP)in the group S1 were significantly increased(P<0.05).The activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC),and the concentrations of reduced glutathione(GSH)and total glutathione(T-GSH)in the groups S1 and S2 were significantly increased(P<0.05),while the content of malondialdehyde(MDA)was significantly decreased(P<0.05).The activities of lysozyme(LZM),total nitric oxide synthase(T-NOS),inducible nitric oxide synthase(iNOS),and nitric oxide(NO)content in the groups S1 and S2 were significantly increased(P<0.05),while the activity of structural nitric oxide synthase(cNOS)was significantly decreased(P<0.05).The AKP activity in the group S1 was significantly higher than that in the group S2(P<0.05).The study shows that fermented feed can promote intestinal development,enhance antioxidant capacity,and immune function in juvenile Ictalurus punctatus.Based on various indicators,the application effects of continuous feeding and interval feeding are comparable.In actual production,interval feeding is sufficient.