浒苔粉替代海带粉对绿盘鲍生长、肠道菌群、消化和抗氧化能力的影响OA

This experiment aimed to investigate the effects of replacing kelp meal with Enteromorpha prolifera meal(EP)on the growth performance,digestive enzyme activities,serum antioxidant,immune indices,and intestinal microbiota structure of green abalones(Haliotis discus hannai ♀×H.fulgens ♂).A total of 168 green abalones with body weight of(34.82±1.38)g were randomly divided into four groups,with three replicates per group and 14 green abalones per replicate.The control group(group CON)was fed a basal diet,and the experimental groups received 10%(group EP10),20%(group EP20),and 30%EP(group EP30)as substitutes for the same level of kelp meal in the basal diet.The pre-trial period lasted for two weeks,and the formal trial period lasted for eight weeks.The results showed that compared with group CON,there were no significant differences in the survival rate(SR),weight gain rate(WGR),specific growth rate(SGR),feed conversion rate(FCR),activities of trypsin,lipase,and α-amylase in the hepatopancreas,and muscle nutrient composition among the experimental groups(P>0.05).In the intestine,Vibrionaceae was enriched in group CON,while Proteobacteria was enriched in group EP30.The content of malondialdehyde(MDA)in the serum of groups EP20 and EP30 was significantly increased(P<0.05).The activities of superoxide dismutase(SOD)and acid phosphatase(ACP)in the serum of group EP30 were significantly decreased(P<0.05).And the activity of aspartate aminotransferase(AST)in group EP20 was significantly increased(P<0.05).The study shows that replacement of kelp meal with EP has no significant effects on digestive enzyme activities,intestinal structure,muscle nutrient composition,and intestinal microbiota diversity and richness of green abalone.Considering all indices comprehensively,it is recommended to control the EP replacement level within 10%in practical applications to balance growth performance and organism health.