饲粮中添加甜菜碱对围产期文登奶山羊瘤胃发酵参数及微生物区系的影响OA

This experiment aimed to investigate the effects of different doses of dietary betaine on the rumen fermentation parameters and microfloras of perinatal Wendeng dairy goats.A total of 48 primiparous Wendeng dairy goats with similar mating time,body weight of(43.93±9.96)kg,and at about three weeks prepartum,were randomly divided into four groups with four replicates per group and three goats per replicate.The control group(group CG)was fed a basal diet,and the experimental groups were fed the basal diet supplemented with 6(group BET1),12(group BET2),and 18 g/d betaine per goat(group BET3),respectively.The pre-test period was seven days,and the formal test period was 50 days.The results showed that compared with group CG,the rumen fluid pH value in group BET1 was significantly decreased(P<0.05),and the rumen fluid acetic acid(AA)content in group BET3 was significantly decreased(P<0.05).Compared with group CG,the relative abundances of RF16 group of Bacteroidetes,vadinBB60 group of Clostridia,and WCHB1-41 in groups BET2 and BET3 were significantly increased(P<0.05).The relative abundances of Anaerolineaceae and Selenomonadaceae in group BET2 were significantly increased(P<0.05).While the relative abundances of the above-mentioned bacterial groups and Selenomonas in group BET1 were significantly decreased(P<0.05).Correlation analysis showed that the WCHB1-41 was extremely positively correlated with ammonia nitrogen(NH3-N)(P<0.01).The Clostridiaceae vadinBB60 group,Bacteroidaceae RF16 group,Clostridium vadinBB60 group,Bacteroides RF16 group were significantly positively correlated with NH3-N(P<0.05).The Butyrivibrio was significantly negatively correlated with isobutyric acid(IBA)(P<0.05).The study shows that dietary supplementation with 12 g/d betaine per goat can optimize rumen fermentation,promote the proliferation of beneficial bacteria.